Borup-Christensen P, Erb K, Jensenius J C
Institute of Surgery, University of Odense, Denmark.
J Immunol Methods. 1988 Jun 13;110(2):237-40. doi: 10.1016/0022-1759(88)90109-3.
Tiamuline and minocycline were evaluated for the treatment of an IgM producing human-human hybridoma cell line infected with Mycoplasma hyorhinis. Tiamuline was used at a concentration of 10 micrograms/ml culture medium and minocycline at a concentration of 5 micrograms/ml culture medium. Both antibiotics were found to eliminate mycoplasma infection over a treatment period of 3 weeks, and the hybridoma cell line remained mycoplasma-free for 6 months after treatment. Tiamuline had no effect on either cell growth or IgM secretion. Whereas treatment with minocycline alternated the cell proliferation and completely inhibited IgM secretion. This effect on cell function was found to be reversible since both cell growth and IgM secretion returned to normal 1 week after the minocycline had been removed. Tiamuline as well as minocycline may be recommended for the treatment of human hybridomas infected with mycoplasma.
评价了泰妙菌素和米诺环素对感染猪鼻支原体的产IgM人-人杂交瘤细胞系的治疗效果。泰妙菌素在培养基中的浓度为10微克/毫升，米诺环素在培养基中的浓度为5微克/毫升。在3周的治疗期内，发现两种抗生素均可消除支原体感染，且治疗后杂交瘤细胞系在6个月内无支原体。泰妙菌素对细胞生长或IgM分泌均无影响。而米诺环素处理则改变了细胞增殖并完全抑制了IgM分泌。由于去除米诺环素1周后细胞生长和IgM分泌均恢复正常，发现这种对细胞功能的影响是可逆的。泰妙菌素和米诺环素均可推荐用于治疗感染支原体的人杂交瘤。
