Arboviruses and Insect Vectors Unit, Institut Pasteur, Paris, France.
Emerging Diseases Epidemiology Unit, Institut Pasteur, Paris, France.
PLoS One. 2021 Apr 1;16(4):e0249471. doi: 10.1371/journal.pone.0249471. eCollection 2021.
Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
当在患者或野外采集的蚊子中检测到虫媒病毒时，就会进行蚊虫控制。然而，大规模筛选蚊子通常既费力又昂贵，需要专业知识和设备。使用浸有蜂蜜的滤纸片检测蚊子唾液中的病毒似乎是一种很有前途的方法，因为它不会造成破坏，并且可以从同一蚊子中随时间监测病毒的排泄动态。在这里，我们在大规模蚊虫监测计划中提出这种方法之前，在实验室条件下测试了使用滤纸片检测蚊子唾液中的基孔肯雅病毒的方法。我们发现，用 50%的蜂蜜溶液浸渍的 0.9 cm2 卡片可以替代强制取食技术，因为它们可以在感染 CHIKV 后的 7 天内检测到 Aedes aegypti 和 Aedes albopictus 蚊子的病毒 RNA。
