Beijing Technology and Business University, Beijing, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing, China.
J Pharm Pharmacol. 2021 Mar 6;73(3):410-423. doi: 10.1093/jpp/rgaa044.
Glochidion ellipticum Wight is a medicinal plant, rich in polyphenols, frequently used by the indigenous communities of Bangladesh and possess with multiple health benefits. It exerts anti-inflammatory and antidiarrheal properties, but the detailed chemical constituents are yet to be elucidated.
Glochidion ellipticum extracts were analyzed using ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and then tested by both lipopolysaccharide (LPS) induced inflammation of Raw 264.7 macrophage cells and dextran sulfate sodium (DSS) induced acute colitis model. Blood serum was taken for fluorescein isothiocyanate-dextran (FITC-dextran) measurement and tissue samples were used to perform histology, RT-PCR and Western blotting.
The extracts could lower the levels of nitric oxide (NO), reactive oxygen species (ROS) and pro-inflammatory cytokines significantly in LPS induced macrophage cells. The extracts could also reduce disease activity index (DAI) score, restore antioxidants and pro-oxidants and improve macroscopic and microscopic features of colonic tissues in DSS induced mice. Expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in protein level was markedly diminished (up to 51.21% and 71.11%, respectively) in the treatment groups compared to the model group of colitic mice.
Our findings suggested that G. ellipticum extracts ameliorate DSS colitis via blocking nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway, which make them to be potential candidates for further research against inflammation and colitis.
黄桐是一种药用植物，富含多酚，常被孟加拉国的土著社区使用，具有多种健康益处。它具有抗炎和抗腹泻作用，但详细的化学成分尚未阐明。
使用超高效液相色谱/四极杆飞行时间质谱对黄桐提取物进行分析，然后通过脂多糖（LPS）诱导的 Raw 264.7 巨噬细胞炎症和葡聚糖硫酸钠（DSS）诱导的急性结肠炎模型进行测试。采集血清进行荧光素异硫氰酸酯-葡聚糖（FITC-dextran）测量，并使用组织样本进行组织学、RT-PCR 和 Western blot 分析。
提取物可显著降低 LPS 诱导的巨噬细胞中一氧化氮（NO）、活性氧（ROS）和促炎细胞因子的水平。提取物还可以降低疾病活动指数（DAI）评分，恢复抗氧化剂和促氧化剂，并改善 DSS 诱导的小鼠结肠组织的宏观和微观特征。与结肠炎模型组相比，治疗组中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白水平表达明显降低（分别降低 51.21%和 71.11%）。
我们的研究结果表明，黄桐提取物通过阻断核因子 kappa-轻链增强子的活化 B 细胞（NF-κB）信号通路改善 DSS 结肠炎，这使其成为进一步研究炎症和结肠炎的潜在候选药物。
