胃癌患者 miR-378c 和 YY1 表达的关系及其与临床病理特征的关系。
Relationship between miR-378c and YY1 expression in patients with gastric cancer and the clinicopathological features.
机构信息
Department of Gastroenterology, Zibo Central Hospital, No. 54 Gongqingtuan West Road, Zhangdian District, Zibo, 255000, Shandong, China.
出版信息
Cell Mol Biol Lett. 2021 Apr 1;26(1):12. doi: 10.1186/s11658-021-00256-x.
BACKGROUND
The purpose of this study was to explore the clinical value of miR-378c and its target gene YY1 in gastric cancer.
METHODS
The TCGA database was employed to analyse miR-378c expression in gastric cancer. qRT-PCR was applied to identify miR-378c and YY1 in tissues and serum of patients suffering from gastric cancer. The association of miR-378c with the clinical data of patients with gastric cancer was analysed. Receiver operating characteristics (ROC) curve analysis was used to determine the diagnostic value of miR-378c and YY1 in gastric cancer, and analyse the relationship between miR-378c and YY1 and patients' survival. Pearson's test was applied to determine the association between miR-378c and YY1 in tissue and serum of patients. Dual-Luciferase Reporter assay was employed to examine the targeting association between miR-378c and YY1. Finally, independent prognostic factors was determined in patients with gastric cancer using Cox regression analysis.
RESULTS
In the TCGA database, miR-378c was weakly expressed in gastric cancer. Overall, patients with low expression had a lower survival rate. The expression of miR-378c decreased and the expression of YY1 increased in cancer tissues and serum of tumour patients. In patients with low expression of miR-378c the tumour size was ≥ 5 cm. Low differentiation, high TNM staging and lymph node invasion rate increased significantly, but the 5-year survival rate decreased in the patients. miR-378c and YY1 had better diagnostic value in gastric cancer. TargetScan, miRDB, starBase and miRTarBase predicted that YY1 was a potential gene of miR-378c, and the Dual-Luciferase Reporter assay revealed that there was a targeting relationship between the two, which was proved by correlation analysis. Multivariate Cox analysis revealed that differentiation, TNM staging and miR-378c were independent prognostic factors for patients.
CONCLUSIONS
MiR-378c is weakly expressed in gastric cancer patients and may be considered as a promising diagnostic and prognostic indicator for gastric cancer.
背景
本研究旨在探讨 miR-378c 及其靶基因 YY1 在胃癌中的临床价值。
方法
利用 TCGA 数据库分析胃癌中 miR-378c 的表达。采用 qRT-PCR 检测胃癌患者组织和血清中 miR-378c 和 YY1 的表达。分析 miR-378c 与胃癌患者临床资料的相关性。采用受试者工作特征（ROC）曲线分析 miR-378c 和 YY1 对胃癌的诊断价值，并分析 miR-378c 与 YY1 与患者生存的关系。采用 Pearson 检验分析 miR-378c 在组织和血清中的相关性。采用双荧光素酶报告基因实验检测 miR-378c 与 YY1 的靶向关系。最后，采用 Cox 回归分析确定胃癌患者的独立预后因素。
结果
在 TCGA 数据库中，miR-378c 在胃癌中表达较弱。总体而言，低表达患者的生存率较低。肿瘤组织和肿瘤患者血清中 miR-378c 表达降低，YY1 表达升高。在 miR-378c 低表达的患者中，肿瘤大小≥5cm。低分化、高 TNM 分期和淋巴结侵犯率显著增加，而患者的 5 年生存率下降。miR-378c 和 YY1 对胃癌具有更好的诊断价值。TargetScan、miRDB、starBase 和 miRTarBase 预测 YY1 是 miR-378c 的潜在靶基因，双荧光素酶报告基因实验证实了两者之间存在靶向关系，相关性分析进一步证实了这一点。多因素 Cox 分析显示，分化、TNM 分期和 miR-378c 是患者的独立预后因素。
结论
miR-378c 在胃癌患者中表达较弱，可能是一种有前途的胃癌诊断和预后指标。
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