哮喘状态诱导了大鼠肺组织中外泌体分泌途径的活性。
Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues.
作者信息
Almohammai Asheed, Rahbarghazi Reza, Keyhanmanesh Rana, Rezaie Jafar, Ahmadi Mahdi
机构信息
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
J Inflamm (Lond). 2021 Apr 1;18(1):14. doi: 10.1186/s12950-021-00275-7.
BACKGROUND
The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma.
RESULTS
Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples (p < 0.05). Along with the promotion of IL-4, the protein level of TNF-α was induced, showing an active inflammatory status in OVA-sensitized rats. According to our data, the promotion of asthmatic responses increased exosome biogenesis indicated by increased CD63 levels and acetylcholine esterase activity compared to the normal condition (p < 0.05).
CONCLUSION
Data suggest that the stimulation of inflammatory response in asthmatic rats could simultaneously increase the paracrine activity of pulmonary cells via the exosome biogenesis. Exosome biogenesis may correlate with the inflammatory response.
背景
近期研究突显了外泌体在炎症调节中的关键作用。在此，我们在哮喘大鼠模型中研究了外泌体的动态生物发生过程。
结果
我们的研究结果显示，与对照样本相比，哮喘肺组织中白细胞介素-4（IL-4）的表达增加，白细胞介素-10（IL-10）受到抑制（p < 0.05）。随着IL-4的增加，肿瘤坏死因子-α（TNF-α）的蛋白水平也被诱导，表明卵清蛋白致敏大鼠处于活跃的炎症状态。根据我们的数据，与正常情况相比，哮喘反应的增强导致外泌体生物发生增加，表现为CD63水平和乙酰胆碱酯酶活性升高（p < 0.05）。
结论
数据表明，哮喘大鼠炎症反应的刺激可通过外泌体生物发生同时增加肺细胞的旁分泌活性。外泌体生物发生可能与炎症反应相关。
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