Zhang Hong, Chen Yan, Cao Dingfang, Li Wenjing, Jing Yanlei, Zhong Hua, Liu Honggang, Zhu Xin
Department of Pathology, Beijing Tongren Hospital, Capital Medical University; Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 100730, China.
These authors contributed equally to this work.
Biomed Opt Express. 2021 Feb 8;12(3):1308-1319. doi: 10.1364/BOE.414931. eCollection 2021 Mar 1.
Laryngeal squamous cell carcinoma (LSCC) is one of the most prevalent malignancy of the upper aerodigestive tract. Detection of early lesions could improve the survival rate significantly. In this study, we demonstrated that femtosecond multiphoton microscopy (MPM) is an effective tool to visualize the microscopic features within fixed laryngeal tissues, without sectioning, staining, or labeling. Accurate detection of lesions and determination of the tumor grading can be achieved, with excellent consistency with conventional histological examination. These results suggest that MPM may represent a powerful tool for in-vivo or fast ex-vivo diagnosis of laryngeal lesions at the point of care.
喉鳞状细胞癌（LSCC）是上消化道最常见的恶性肿瘤之一。早期病变的检测可显著提高生存率。在本研究中，我们证明了飞秒多光子显微镜（MPM）是一种有效的工具，可在不进行切片、染色或标记的情况下可视化固定喉组织内的微观特征。能够实现病变的准确检测和肿瘤分级的确定，与传统组织学检查具有极好的一致性。这些结果表明，MPM可能是一种在床旁对喉部病变进行体内或快速离体诊断的强大工具。
