Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350007, China.
Department of Breast Surgery, Fujian Medical University Union Hospital, Fuzhou 350001, China.
Scanning. 2020 Apr 2;2020:9670514. doi: 10.1155/2020/9670514. eCollection 2020.
Breast cancer can be cured by early diagnosis. Appropriate and effective clinical treatment benefits from accurate pathological diagnosis. However, due to the lack of effective screening and diagnostic imaging methods, early stages of breast cancer often progress to malignant breast cancer. In this study, multiphoton microscopy (MPM) via two-photon excited fluorescence combined with second-harmonic generation was used for identifying the early stages of breast ductal carcinoma. The results showed differences in both cytological features and collagen distribution among normal breast tissue, atypical ductal hyperplasia, low-grade ductal carcinoma in situ, and high-grade ductal carcinoma in situ with microinvasion. Furthermore, three features extracted from the MPM images were used to describe differences in cytological features, collagen density, and basement membrane circumference in the early stages of breast ductal carcinoma. They revealed that MPM has the ability to identify early stages of breast ductal carcinoma label-free, which would contribute to the early diagnosis and treatment of breast cancer. This study may provide the groundwork for the further application of MPM in the clinic.
早期诊断可治愈乳腺癌。适当且有效的临床治疗受益于准确的病理诊断。然而，由于缺乏有效的筛查和诊断成像方法，乳腺癌的早期阶段常进展为恶性乳腺癌。在这项研究中，采用双光子激发荧光结合二次谐波产生的多光子显微镜（MPM）来识别乳腺导管癌的早期阶段。结果表明，在正常乳腺组织、非典型导管增生、低级别导管原位癌和微浸润高级别导管原位癌之间，细胞特征和胶原分布存在差异。此外，从 MPM 图像中提取的三个特征用于描述乳腺导管癌早期阶段细胞特征、胶原密度和基膜周长的差异。结果表明 MPM 具有无标记识别乳腺导管癌早期阶段的能力，这有助于乳腺癌的早期诊断和治疗。本研究可为 MPM 在临床上的进一步应用奠定基础。
