D'Amico Francesco, Bertacco Alessandra, Finotti Michele, Di Renzo Chiara, Rodriguez-Davalos Manuel I, Gondolesi Gabriel E, Cillo Umberto, Mulligan David, Geibel John
Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, Oncology and Gastroenterology, Padua University, Padua, Italy.
Transplantation and Immunology Section, Department of Surgery, Yale University, New Haven, CT, United States.
Front Surg. 2021 Mar 16;8:621525. doi: 10.3389/fsurg.2021.621525. eCollection 2021.
Historically, bile in the biliary tract has been considered sterile. Most of the series are based on patients with biliary tract diseases or the bile has been obtained with procedures susceptible to contamination. We evaluated the bile in a heterogeneous cohort of liver donors and recipient patients, with samples obtained in a sterile way, directly from the gallbladder and the common bile duct. We assessed the bile microbiota in six liver donors and in six liver recipients after whole or split liver procedures in adult or pediatric recipients. Bile samples were studied using PCR sequencing of the 16S ribosomal RNA gene amplification (rDNA). We demonstrated that the bile is sterile, thereby ruling this out as a source of contamination following transplant.
从历史上看，胆道中的胆汁一直被认为是无菌的。大多数研究系列都是基于患有胆道疾病的患者，或者胆汁是通过易受污染的操作获取的。我们评估了一组异质性的肝脏供体和受体患者的胆汁，样本通过无菌方式直接从胆囊和胆总管获取。我们评估了6名肝脏供体以及6名接受全肝或劈离式肝移植手术的成年或儿童受体患者术后的胆汁微生物群。使用16S核糖体RNA基因扩增（rDNA）的PCR测序对胆汁样本进行研究。我们证明胆汁是无菌的，从而排除了其作为移植后污染来源的可能性。
