胆管结石或远端胆管癌患者胆道微生物群的初步研究。
A Preliminary Study of Biliary Microbiota in Patients with Bile Duct Stones or Distal Cholangiocarcinoma.
机构信息
Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 200080, China.
出版信息
Biomed Res Int. 2019 Sep 25;2019:1092563. doi: 10.1155/2019/1092563. eCollection 2019.
BACKGROUND AND OBJECTIVE
The distal cholangiocarcinoma (dCCA) is associated with many factors: genes, environment, infection, etc. The current changes in biliary flora are thought to be involved in the formation of many gastrointestinal tract (GIT) diseases, like colon adenocarcinoma. Therefore we want to investigate whether the dCCA has a certain correlation with biliary microecology, and to detect specific strains.
METHODS
A total of 68 adults were enrolled, of whom 8 with dCCA, 16 with recurrent choledocholithiasis, and 44 with the onset of common bile duct stones. Endoscopic Retrograde Cholangiopancretography (ERCP) was utilized to collect bile samples for DNA extraction and 16S rRNA gene sequencing, followed by analysis of bile microbiota composition.
RESULTS
First, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria are the most dominant phyla in the bile of patients with dCCA and the onset of common bile duct stoes. Secondly, compared with the onset of common bile duct stones patients, we got a significant increase in the phylum Gemmatimonadetes, Nitrospirae, Chloroflexi, Latescibacteria, and Planctomycetes in dCCA patients. Finally, at the genus level, we obtained sequencing results of 252 bacterial genera from patients with dCCA, recurrent choledocholithiasis, and the new onset of common bile duct stones, revealing heterogeneity among individuals.
CONCLUSION
To the best of our knowledge, this is the first study of the dysbiosis of bile flora in patients with dCCA. This micro-ecological disorder may be a decisive factor in the formation of dCCA. At the same time, for the first time, this study provides a test chart of biliary microbial populations that may be associated with recurrent choledocholithiasis. The compositional changes of the core microbial group of the biliary tract have potentially important biological and medical significance for the microbiological biliary disorders of dCCA.
背景与目的
远端胆管癌（dCCA）与许多因素有关：基因、环境、感染等。目前认为胆道菌群的变化与许多胃肠道（GIT）疾病有关，如结肠癌。因此，我们想研究 dCCA 是否与胆道微生态有一定的相关性，并检测特定的菌株。
方法
共纳入 68 例成年人，其中 8 例为 dCCA，16 例为复发性胆总管结石，44 例为胆总管结石初发。经内镜逆行胰胆管造影术（ERCP）收集胆汁样本进行 DNA 提取和 16S rRNA 基因测序，分析胆汁微生物群落组成。
结果
首先，在 dCCA 和胆总管结石初发患者的胆汁中，变形菌门、厚壁菌门、拟杆菌门和放线菌门是最主要的菌门。其次，与胆总管结石初发患者相比，dCCA 患者的芽单胞菌门、硝化螺旋菌门、绿弯菌门、浮霉菌门和计划菌门显著增加。最后，在属水平上，我们从 dCCA、复发性胆总管结石和胆总管结石初发患者中获得了 252 个细菌属的测序结果，显示个体之间存在异质性。
结论
据我们所知，这是第一项关于 dCCA 患者胆汁菌群失调的研究。这种微生态失调可能是 dCCA 形成的决定性因素。同时，本研究首次提供了与复发性胆总管结石相关的胆道微生物群的检测图谱。胆道核心微生物群的组成变化对 dCCA 的微生物胆道疾病具有潜在的重要生物学和医学意义。
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