Niu Ruichang, Gao Xueke, Luo Junyu, Wang Li, Zhang Kaixin, Li Dongyang, Ji Jichao, Cui Jinjie, Zhu Xiangzhen, Zhang Shuai
Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang, Henan, China.
School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu, China.
Mitochondrial DNA B Resour. 2021 Mar 16;6(3):922-924. doi: 10.1080/23802359.2021.1888328.
The complete mitochondrial genome of Glover cucumber biotype was sequenced using traditional PCR amplification coupled with Sanger sequencing. The genome is 15,870 bp long, with 83.7% AT content (MW048625). The genome encodes 37 typical mitochondrial genes, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, a repeat region of 784 bp, and a control region of 627 bp. The base composition of the genome is A (45.4%), T (38.3%), C (10.5%), and G (5.8%). An analysis of two biotypes mitogenomes identified 77 single nucleotide polymorphisms and 1 insertion and deletion.
利用传统PCR扩增结合桑格测序法对格洛弗黄瓜生物型的完整线粒体基因组进行了测序。该基因组长度为15,870 bp，AT含量为83.7%（MW048625）。该基因组编码37个典型的线粒体基因，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转移RNA、一个784 bp的重复区域和一个627 bp的控制区域。基因组的碱基组成为A（45.4%）、T（38.3%）、C（10.5%）和G（5.8%）。对两种生物型线粒体基因组的分析鉴定出77个单核苷酸多态性以及1个插入缺失。
