Jang Yo-Soon, Lee Eun Kyung, Kim Sung
a East Sea Environment Research Division , Korea Institute of Ocean Science & Technology , Uljin-gun , Gyeongbuk , Republic of Korea .
b Department of Convergence Study on the Ocean Science and Technology , Ocean Science and Technology School, Korea Maritime and Ocean University , Busan , Republic of Korea .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):1970-2. doi: 10.3109/19401736.2014.971311. Epub 2014 Oct 20.
Hybrids of Sebastes hubbsi, considered a valid species, have been found in the ocean. To examine the basic characteristics of mitochondrial DNA, we analysed the complete mitogenome of S. hubbsis using next-generation sequencing. The complete mitogenome was 16,453 bp in length and comprised 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and 1 control region. The complete mitochondrial DNA had 27.9% A, 26.7% T, 17.2% G and 28.3% C, showing a slight AT bias (54.5%). The start codon of all PCGs was ATG, except COX1 (GTG). The stop codons in the PCGs were mainly TAA, except ND1 and ND3 (TAG), ND4 (AGA) and Cytb (incomplete termination codon, T). The tRNAs had a typical cloverleaf structure, except tRNA(Ser (AGY)) which lacked the DHU arm. The complete mitogenome of S. hubbsi can be used for hybridization or on-going speciation studies within allied species in the Scorpaenidae.
被视为一个有效物种的赫氏平鲉(Sebastes hubbsi)的杂交种已在海洋中被发现。为了研究线粒体DNA的基本特征,我们使用下一代测序技术分析了赫氏平鲉的完整线粒体基因组。完整的线粒体基因组长度为16,453 bp,由13个蛋白质编码基因(PCGs)、2个rRNA、22个tRNA和1个控制区组成。完整的线粒体DNA含有27.9%的A、26.7%的T、17.2%的G和28.3%的C,显示出轻微的AT偏好(54.5%)。除COX1(GTG)外,所有PCGs的起始密码子均为ATG。PCGs中的终止密码子主要是TAA,除了ND1和ND3(TAG)、ND4(AGA)以及Cytb(不完全终止密码子,T)。tRNAs具有典型的三叶草结构,除了tRNA(Ser (AGY))缺少DHU臂。赫氏平鲉的完整线粒体基因组可用于鲉科相关物种内的杂交或正在进行的物种形成研究。