甘薯（Ipomoea batatas L.）中一种新型叶绿素缺乏突变体（）的完整叶绿体基因组
Complete chloroplast genome of a novel chlorophyll-deficient mutant () in sweetpotato ( L.).
作者信息
Zou Hongda, Zhang Xiongjian, Chen Jingyi, Wang Zhangying, Huang Lifei, Fang Boping
机构信息
Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
出版信息
Mitochondrial DNA B Resour. 2021 Mar 18;6(3):968-969. doi: 10.1080/23802359.2020.1869616.
The complete chloroplast genome of a novel chlorophyll-deficient mutant () and its wild type (WT) in sweetpotato ( L.) was sequenced. The complete chloroplast genome of and WT was 161,393 bp and 161,429 bp in length, containing a large single copy (LSC) region of 87,561 bp and 87,597 bp, respectively, a small single copy (SSC) region with the same length of 30,890 bp and a pair of inverted repeat regions (IRs) with the same length of 12,052 bp. Both of them contained 132 genes including 87 protein-coding sequences, 37 tRNA, and eight rRNA. Comparing to the WT, four SNPs and three INDELs were detected and only one INDEL in the exon affecting the translation of gene. Phylogenetic analysis showed that and WT were closely related to . The complete chloroplast genome of and its WT will play a role in understanding the molecular mechanism of chlorophyll deficiency and developing molecular markers in sweetpotato.
对甘薯（Ipomoea batatas L.）中一个新的叶绿素缺乏突变体（）及其野生型（WT）的完整叶绿体基因组进行了测序。突变体和野生型的完整叶绿体基因组长度分别为161,393 bp和161,429 bp，包含一个长度为87,561 bp和87,597 bp的大单拷贝（LSC）区域、一个长度均为30,890 bp的小单拷贝（SSC）区域以及一对长度均为12,052 bp的反向重复区域（IR）。它们都包含132个基因，包括87个蛋白质编码序列、37个tRNA和8个rRNA。与野生型相比，检测到4个单核苷酸多态性（SNP）和3个插入缺失（INDEL），且外显子中只有一个INDEL影响基因的翻译。系统发育分析表明，突变体和野生型与[具体品种]密切相关。突变体及其野生型的完整叶绿体基因组将有助于理解甘薯叶绿素缺乏的分子机制并开发分子标记。
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