Wang Qian, Hu Huan, An Jiaxing, Bai Guohui, Ren Qunli, Liu Jianguo
Research Center for Medicine & Biology, Zunyi Medical University, Zunyi, China.
Department of Gastroenterology, Affiliated Hospital, Zunyi Medical University, Zunyi, China.
Mitochondrial DNA B Resour. 2018 Feb 1;3(1):149-150. doi: 10.1080/23802359.2018.1431074.
Tratt. is a famous Chinese traditional medicine with long history, here we characterized the whole chloroplast (cp) genome sequence of by Illumina pair-end sequencing. The complete cp genome was 156,749 bp in length, containing a large single copy (LSC) region of 85,862 bp and a small single copy (SSC) region of 18,791 bp, which were separated by a pair of 26,053 bp inverted repeat regions (IRs). The genome contained 139 genes, including 88 protein-coding genes (82 PCG species), 39 tRNA genes (32 tRNA species), and eight ribosomal RNA genes (four rRNA species). Most of the gene species occur as a single copy, while 17 gene species occur in double copies. The overall AT content of cp genome is 62.8%, while the corresponding values of the LSC, SSC, and IR regions are 64.8, 68.7, and 57.4%, respectively. Further, phylogenetic analysis suggested that is closely related to var. .
Tratt.是一种历史悠久的著名中药,在此我们通过Illumina双末端测序对其叶绿体(cp)全基因组序列进行了特征分析。完整的cp基因组长度为156,749 bp,包含一个85,862 bp的大单拷贝(LSC)区域和一个18,791 bp的小单拷贝(SSC)区域,它们被一对26,053 bp的反向重复区域(IRs)隔开。该基因组包含139个基因,包括88个蛋白质编码基因(82种PCG)、39个tRNA基因(32种tRNA)和8个核糖体RNA基因(4种rRNA)。大多数基因种类以单拷贝形式存在,而17种基因种类以双拷贝形式存在。Tratt. cp基因组的总体AT含量为62.8%,而LSC、SSC和IR区域的相应值分别为64.8%、68.7%和57.4%。此外,系统发育分析表明Tratt.与var.密切相关。