Ruggieri M R, Levin R M, Hanno P M, Witkowski B A, Gill H S, Steinhardt G F
Division of Urology, University of Pennsylvania School of Medicine, Philadelphia.
J Urol. 1988 Jul;140(1):157-9. doi: 10.1016/s0022-5347(17)41517-5.
A substantial body of animal work indicates that the initial first line defense against invading microorganisms in the urinary tract is the antiadherence activity of the surface mucin layer. Previous work has demonstrated that bacterial adherence to anion exchange resin can be used as a model for adherence to the mucin deficient rabbit bladder. This anion exchange resin adherence model can also be used as a rapid screen for potential antiadherence agents. In vitro saline extracts of bladder mucosa from various mammalian species including man have been shown to inhibit bacterial adherence to both anion exchange resin and the mucin deficient rabbit bladder. The present report investigates the ability of in vivo saline bladder washes from several groups of patients to inhibit bacterial adherence to anion exchange resin. This has an advantage over other methods of quantitation or visualization of mucin since it is the ability of the bladder extract to prevent bacterial adherence, and not merely the quantity of mucin, that determines the effectiveness of the mucin lining in preventing bacterial attachment. Bladder washes from patients with recurrent urinary tract infection were significantly less potent at inhibiting bacterial adherence than extracts from other groups of patients. This decreased functional antiadherence activity of bladder extracts may help explain the frequency of urinary tract infection in this group of patients.
大量动物实验表明，泌尿道抵御入侵微生物的第一道防线是表面粘蛋白层的抗黏附活性。先前的研究表明，细菌对阴离子交换树脂的黏附可作为其对黏蛋白缺乏的兔膀胱黏附的模型。这种阴离子交换树脂黏附模型也可用于快速筛选潜在的抗黏附剂。包括人类在内的各种哺乳动物膀胱黏膜的体外盐水提取物已被证明能抑制细菌对阴离子交换树脂和黏蛋白缺乏的兔膀胱的黏附。本报告研究了几组患者体内膀胱盐水冲洗液抑制细菌对阴离子交换树脂黏附的能力。这一方法相对于其他黏蛋白定量或可视化方法具有优势，因为决定黏蛋白内衬预防细菌附着有效性的是膀胱提取物防止细菌黏附的能力，而不仅仅是黏蛋白的量。复发性尿路感染患者的膀胱冲洗液在抑制细菌黏附方面的效力明显低于其他患者组的提取物。膀胱提取物这种功能性抗黏附活性的降低可能有助于解释该组患者尿路感染的频发情况。
