Ruggieri M R, Hanno P M, Levin R M
Urol Res. 1984;12(4):199-203. doi: 10.1007/BF00256803.
Previous studies performed in our laboratory have indicated that the primary antibacterial defense mechanism of the rabbit urinary bladder is the antiadsorptive action of the surface mucopolysaccharide. Removal of this layer with an acid rinse increases bacterial adherence up to 100 fold. Exogenous mucopolysaccharide (heparin) has been shown to restore Escherichia coli adherence to control levels. To determine whether this antiadherence action of heparin is species specific, we compared the adherence of 5 common urinary tract pathogens (Escherichia coli, Klebsiella ozonae, Proteus mirabilis, Pseudomonas aeruginosa, and Streptococcus fecalis) to both mucin intact and mucin deficient rabbit bladders with and without prior heparin exposure. Bacteria were radiolabeled by addition of 3H-adenine to the culture broth so that the number of bacteria adhering to the bladder could be determined using liquid scintillation spectrophotometry. Results were as follows: Acid removal of the mucin layer significantly increased the adherence approximately 10 fold for all 5 species tested. Briefly exposing the mucin deficient bladders to heparin decreased the adherence of all species tested except Pseudomonas to mucin intact control levels. Heparin treatment of mucin intact bladders slightly decreased adherence of all species except Pseudomonas below mucin intact controls, however, results were not statistically significant. The magnitude of Klebsiella adherence was nearly 20 fold greater than all other species tested. While this non-species specific adherence inhibition of heparin may prove useful in the clinical setting, it appears to be less effective against Pseudomonas.
我们实验室之前进行的研究表明，兔膀胱的主要抗菌防御机制是表面粘多糖的抗吸附作用。用酸冲洗去除这一层会使细菌黏附增加至100倍。已证明外源性粘多糖（肝素）可将大肠杆菌的黏附恢复至对照水平。为了确定肝素的这种抗黏附作用是否具有物种特异性，我们比较了5种常见尿路病原体（大肠杆菌、臭鼻克雷伯菌、奇异变形杆菌、铜绿假单胞菌和粪链球菌）在有或无肝素预先处理的情况下，对粘蛋白完整和粘蛋白缺乏的兔膀胱的黏附情况。通过向培养液中添加³H-腺嘌呤对细菌进行放射性标记，以便使用液体闪烁分光光度法测定黏附在膀胱上的细菌数量。结果如下：酸去除粘蛋白层后，所有5种测试菌的黏附均显著增加约10倍。将粘蛋白缺乏的膀胱短暂暴露于肝素下，除铜绿假单胞菌外，所有测试菌的黏附均降至粘蛋白完整对照水平。肝素处理粘蛋白完整的膀胱后，除铜绿假单胞菌外，所有菌的黏附均略低于粘蛋白完整对照，但结果无统计学意义。克雷伯菌的黏附程度比所有其他测试菌几乎高20倍。虽然肝素这种非物种特异性的黏附抑制作用在临床环境中可能有用，但它对铜绿假单胞菌的效果似乎较差。
