Pham Christina Van Anh, Rademacher Franziska, Hinrichs Heilwig, Beck-Jendroschek Vera, Harder Melanie, Brasch Jochen, Gläser Regine, Harder Jürgen
Department of Dermatology, Venerology and Allergology, Quincke Research Center, University-Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Department of Dermatology, Venerology and Allergology, Mycological Laboratory, University Hospitals of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Mycoses. 2021 Jul;64(7):763-770. doi: 10.1111/myc.13279. Epub 2021 May 4.
Tinea pedis is often chronic or recurrent, but not all individuals are equally susceptible to this infection. Dermatophytes are able to induce the expression of antimicrobial peptides and proteins (AMPs) in human keratinocytes and certain AMPs can inhibit the growth of dermatophytes.
The focus of this study was to analyse the secretion of relevant AMPs, especially RNase 7, human beta-defensin-2 (hBD-2) and the S-100 protein psoriasin (S100A7), in patients with confirmed tinea pedis.
To verify the diagnosis, skin scales were obtained from all patients (n = 13) and the dermatophytes were identified by potassium hydroxide mount, culture and molecular analysis. To determine the AMP concentrations, the lesional skin area of the foot was rinsed with a buffer that was subsequently analysed by ELISA. The corresponding area of the other unaffected foot as well as defined healthy skin areas of the forearm and forehead and samples from age and gender-matched healthy volunteers served as controls.
In tinea pedis patients the AMP concentrations were higher in lesional skin than in non-lesional skin and in healthy skin of controls. In particular, concentrations of hBD-2 and psoriasin were significantly elevated.
The induction of AMPs in tinea pedis might be triggered directly by the dermatophytes; furthermore, attendant inflammation and/or differentiation processes may play a role. Our results indicate that there is no defect in the constitutive expression and induction of the analysed AMPs by dermatophytes in the epidermis of affected patients. However, it is not known why the elevated AMP concentrations fail to efficiently combat dermatophyte growth.
足癣通常为慢性或复发性疾病，但并非所有人都同样易患这种感染。皮肤癣菌能够诱导人角质形成细胞中抗菌肽和蛋白质（AMPs）的表达，某些AMPs可抑制皮肤癣菌的生长。
本研究的重点是分析确诊为足癣的患者中相关AMPs的分泌情况，尤其是核糖核酸酶7、人β-防御素-2（hBD-2）和S-100蛋白银屑素（S100A7）。
为了确诊，从所有患者（n = 13）处获取皮肤鳞屑，通过氢氧化钾涂片、培养和分子分析鉴定皮肤癣菌。为了测定AMPs浓度，用缓冲液冲洗足部皮损区皮肤，随后通过酶联免疫吸附测定（ELISA）进行分析。另一只未受影响足部的相应区域以及前臂和前额明确的健康皮肤区域，以及年龄和性别匹配的健康志愿者的样本作为对照。
足癣患者皮损区皮肤中的AMPs浓度高于非皮损区皮肤以及对照的健康皮肤。特别是，hBD-2和银屑素的浓度显著升高。
足癣中AMPs的诱导可能直接由皮肤癣菌触发；此外，伴随的炎症和/或分化过程可能起作用。我们的结果表明，在受影响患者的表皮中，所分析的AMPs的组成性表达和由皮肤癣菌诱导的过程没有缺陷。然而，尚不清楚为什么升高的AMPs浓度未能有效对抗皮肤癣菌的生长。
