Department of Biosystems Engineering, Institute of Forest Science, Kangwon National University, Chuncheon, Republic of Korea.
Department of Biological and Agricultural Engineering, Institute for Nanoscience and Engineering, University of Arkansas, Fayetteville, Arkansas, USA.
J Biomed Mater Res A. 2021 Oct;109(10):1869-1880. doi: 10.1002/jbm.a.37179. Epub 2021 Apr 2.
Carbon nanotubes (CNTs) have attracted significant interest for various applications owing to their superior physicochemical properties. The unzipping of multi-walled carbon nanotubes was accomplished by strong acid treatment. The solution of unzipped carbon nanotubes (u-CNTs) was homogeneous and stable. The u-CNTs were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis. The dimensions and morphologies of the synthesized u-CNTs were examined by transmission electron microscopy and scanning electron microscopy. The u-CNTs exhibited increased zeta potential and diameter compared with pure CNTs. A decrease in the thermal stability was observed in the u-CNTs compared with pure CNTs. The u-CNTs exhibited better biocompatibility than pure CNTs in the presence of bone marrow-derived mesenchymal stem cells, showing improved biocompatibility. The u-CNT-treated media generated lower amounts of reactive oxygen species than pure CNTs. Enhanced mineralization was observed in the u-CNT-treated groups compared with the pure CNTs and the control, indicating its better osteogenic potential. The upregulation of osteogenic-associated gene markers in u-CNT groups compared with pure CNTs confirms their superior osteogenic potential. Thus, u-CNTs are potential candidates for tissue engineering applications, especially bone tissue.
碳纳米管（CNTs）由于其优异的物理化学性质而引起了广泛关注。通过强酸处理实现了多壁碳纳米管的解卷。解卷碳纳米管（u-CNTs）的溶液是均相和稳定的。通过傅里叶变换红外光谱、X 射线衍射和热重分析对 u-CNTs 进行了表征。通过透射电子显微镜和扫描电子显微镜检查了合成的 u-CNTs 的尺寸和形态。与纯 CNTs 相比，u-CNTs 表现出更高的 Zeta 电位和直径。与纯 CNTs 相比，u-CNTs 的热稳定性下降。在骨髓间充质干细胞存在的情况下，u-CNTs 表现出比纯 CNTs 更好的生物相容性，显示出更好的生物相容性。与纯 CNTs 和对照相比，u-CNT 处理的培养基中产生的活性氧物质较少。与纯 CNTs 和对照相比，u-CNT 处理组观察到增强的矿化，表明其具有更好的成骨潜力。与纯 CNTs 相比，u-CNT 组中与成骨相关的基因标志物上调，证实了其更好的成骨潜力。因此，u-CNTs 是组织工程应用的潜在候选材料，特别是在骨组织中。
