Guangxi Key Lab of Sugarcane Biology, State Key Lab for Conservation and Utilization of Sub-tropical Agri-Biological Resources, Guangxi University, Nanning, 530005, China.
Nuclear Institute of Agriculture (NIA), Tando Jam, 70060, Pakistan.
Mol Plant Microbe Interact. 2021 Aug;34(8):973-976. doi: 10.1094/MPMI-11-20-0331-A. Epub 2021 Aug 24.
Sugarcane pokkah boeng disease (PBD) is emerging as a prevalent foliar disease in China. This airborne disease is caused by the species complex. To investigate the diversity and evolution of spp., we performed whole-genome sequencing of YN28 using a combination of Oxford Nanopore and Illumina technology. The YN28 genome was sequenced, assembled, and annotated. A high-quality genome was assembled into 24 contigs with an N of 2.80 Mb. The genome assembly generated a total size of 44.1 Mb with a GC content of 47.64%. In total, 15,508 genes were predicted, including 794 genes related to the carbohydrate-active enzymes, 397 noncoding RNA, 155 genes associated with transporter classification, 4,550 genes linked to pathogen-host interactions, and 269 genes involved in effector proteins. Collectively, our results will provide insight into the host-pathogen interactions and will facilitate the breeding of new varieties of sugarcane resistant to PBD.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
甘蔗曲叶病（PBD）在中国正逐渐成为一种流行的叶部病害。这种气传疾病是由 种复合体引起的。为了研究 spp. 的多样性和进化，我们使用 Oxford Nanopore 和 Illumina 技术组合对 YN28 进行了全基因组测序。对 YN28 进行了测序、组装和注释。高质量的基因组被组装成 24 个 contigs，N 值为 2.80 Mb。基因组组装总共产生了 441 Mb 的大小，GC 含量为 47.64%。总共预测了 15508 个基因，包括 794 个与碳水化合物活性酶相关的基因、397 个非编码 RNA、155 个与转运蛋白分类相关的基因、4550 个与病原体-宿主相互作用相关的基因和 269 个与效应蛋白相关的基因。总的来说，我们的研究结果将深入了解寄主-病原体相互作用，并有助于培育新的抗 PBD 的甘蔗品种。
