Inayama Y, Hook G E, Brody A R, Cameron G S, Jetten A M, Gilmore L B, Gray T, Nettesheim P
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina.
Lab Invest. 1988 Jun;58(6):706-17.
The objective of this study was to examine the differentiation potential of basal cells purified from rabbit tracheas by means of centrifugal elutriation. Cell suspensions containing between 83 and 94% basal cells were inoculated into tracheal grafts denuded of their own epithelium and transplanted to the backs of nude mice. Within 1 week after inoculation, the basal cells reestablished a continuous poorly differentiated epithelial sheet which at 2 to 4 weeks showed typical mucociliary differentiation. Basal cells, ciliated cells, goblet cells, and secretory cells with small granules were present. Some of the latter contained abundant rough endoplasmic reticulum and others contained predominantly smooth endoplasmic reticulum. These data indicate that basal cells can give rise to all major tracheal cell types suggesting that they are the main stem cell in the large airways of rabbits.
本研究的目的是通过离心淘析法检测从兔气管中纯化的基底细胞的分化潜能。将含有83%至94%基底细胞的细胞悬液接种到去除自身上皮的气管移植物中，并移植到裸鼠背部。接种后1周内，基底细胞重新形成连续的低分化上皮片，在2至4周时显示出典型的黏液纤毛分化。存在基底细胞、纤毛细胞、杯状细胞和含小颗粒的分泌细胞。后者中的一些含有丰富的粗面内质网，另一些则主要含有滑面内质网。这些数据表明，基底细胞可分化为所有主要的气管细胞类型，提示它们是兔大气道中的主要干细胞。
