利用 CRISPR/Cas9 编辑技术生成 TLR2 纯合敲除的人类胚胎干细胞系 WAe001-A-64。
Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing.
机构信息
Institute of Public Health, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of China Academy of Sciences, Beijing 100049, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Institute of Public Health, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
出版信息
Stem Cell Res. 2021 Jul;54:102401. doi: 10.1016/j.scr.2021.102401. Epub 2021 May 21.
Toll-like receptor 2 (TLR2) is a pattern recognition receptor which plays an important role in innate immune system. In humans it's encoded by the TLR2 gene and also been designated as CD282. Using CRISPR/Cas9 gene editing technology, we have established a TLR2 mutant WAe001-A-64 cell line from the original embryonic stem cell line H1. It has adopted two biallelic deletions in exon 3 of TLR2 which resulted in a frame shift and early termination in the translation of TLR2. Moreover, WAe001-A-64 has maintained the normal karyotype, pluripotent phenotype, parental cell morphology and the ability to differentiate into three germ layers.
Toll-like receptor 2 (TLR2) 是一种模式识别受体，在先天免疫系统中发挥重要作用。在人类中，它由 TLR2 基因编码，也被指定为 CD282。我们使用 CRISPR/Cas9 基因编辑技术，从原始胚胎干细胞系 H1 中建立了 TLR2 突变体 WAe001-A-64 细胞系。该细胞系在 TLR2 的外显子 3 中采用了两个双等位基因缺失，导致 TLR2 的翻译发生移码和提前终止。此外，WAe001-A-64 保持了正常的核型、多能表型、亲本细胞形态和分化为三个胚层的能力。
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