诱导多能干细胞系 (iPSTi002-A) 的建立，来源于一位 87 岁散发性阿尔茨海默病患者，其 APOE 基因型为 APOE3 (ε3/ε3)。
Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype.
机构信息
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China; Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
出版信息
Stem Cell Res. 2021 May;53:102282. doi: 10.1016/j.scr.2021.102282. Epub 2021 Mar 12.
Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis.
在这里，我们描述了从一位 87 岁患有散发性阿尔茨海默病（sAD）的女性患者外周血单个核细胞（PBMCs）中生成人诱导多能干细胞（iPSC）的过程。该患者的 APOE3（ε3/ε3）基因型。我们使用附加体系统，通过 OCT4、SOX2、c-MYC 和 KLF4 四种因子从 PBMCs 中生成 iPSC 系。通过胚状体（EB）形成评估 iPSC 系的多能性。流式细胞术分析显示，超过 97%的细胞对多能性标志物 NANOG、OCT4 和 SSEA4 呈阳性。此外，iPSC 系还显示出正常的核型（46，XX）。该 iPSC 系可能为研究 sAD 发病机制提供有价值的工具。
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