Buchroithner Boris, Spurný Pavel, Mayr Sandra, Heitz Johannes, Sivun Dmitry, Jacak Jaroslaw, Ludwig Jost
School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria, Garnisonstr. 21, 4020 Linz, Austria.
Institute of Microbiology of the Czech Academy of Sciences, Zamek 136, 37333 Nove Hrady, Czech Republic.
Micromachines (Basel). 2021 Mar 6;12(3):273. doi: 10.3390/mi12030273.
The microelectrode ion flux estimation (MIFE) is a powerful, non-invasive electrophysiological method for cellular membrane transport studies. Usually, the MIFE measurements are performed in a tissue culture dish or directly with tissues (roots, parts of the plants, and cell tissues). Here, we present a transwell system that allows for MIFE measurements on a cell monolayer. We introduce a measurement window in the transwell insert membrane, which provides direct access for the cells to the media in the upper and lower compartment of the transwell system and allows direct cell-to-cell contact coculture. Three-dimensional multiphoton lithography (MPL) was used to construct a 3D grid structure for cell support in the measurement window. The optimal polymer grid constant was found for implementation in transwell MIFE measurements. We showed that human umbilical vein endothelial cells (HUVECs) efficiently grow and maintain their physiological response on top of the polymer structures.
微电极离子通量估算（MIFE）是一种用于细胞膜运输研究的强大的非侵入性电生理方法。通常，MIFE测量是在组织培养皿中或直接对组织（根、植物的部分以及细胞组织）进行的。在此，我们展示了一种能够对细胞单层进行MIFE测量的Transwell系统。我们在Transwell插入膜中引入了一个测量窗口，该窗口为细胞提供了直接接触Transwell系统上下腔室中培养基的通道，并允许细胞间直接接触共培养。使用三维多光子光刻（MPL）在测量窗口中构建用于细胞支撑的三维网格结构。找到了用于Transwell MIFE测量的最佳聚合物网格常数。我们证明了人脐静脉内皮细胞（HUVECs）能够在聚合物结构顶部高效生长并维持其生理反应。
