Rovira-Llopis Susana, Díaz-Rúa Rubén, Grau-Del Valle Carmen, Iannantuoni Francesca, Abad-Jimenez Zaida, Bosch-Sierra Neus, Panadero-Romero Joaquín, Victor Víctor M, Rocha Milagros, Morillas Carlos, Bañuls Celia
Service of Endocrinology, University Hospital Doctor Peset, Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (FISABIO), 46017 Valencia, Spain.
Genomic Unit, La Fe Health Research Institute, 46026 Valencia, Spain.
Biomedicines. 2021 Mar 21;9(3):321. doi: 10.3390/biomedicines9030321.
Obese individuals without metabolic comorbidities are categorized as metabolically healthy obese (MHO). MicroRNAs (miRNAs) may be implicated in MHO. This cross-sectional study explores the link between circulating miRNAs and the main components of metabolic syndrome (MetS) in the context of obesity. We also examine oxidative stress biomarkers in MHO vs. metabolically unhealthy obesity (MUO). We analysed 3536 serum miRNAs in 20 middle-aged obese individuals: 10 MHO and 10 MUO. A total of 159 miRNAs were differentially expressed, of which, 72 miRNAs (45.2%) were higher and 87 miRNAs (54.7%) were lower in the MUO group. In addition, miRNAs related to insulin signalling and lipid metabolism pathways were upregulated in the MUO group. Among these miRNAs, hsa-miR-6796-5p and hsa-miR-4697-3p, which regulate oxidative stress, showed significant correlations with glucose, triglycerides, HbA1c and HDLc. Our results provide evidence of a pattern of differentially expressed miRNAs in obesity according to MetS, and identify those related to insulin resistance and lipid metabolism pathways.
没有代谢合并症的肥胖个体被归类为代谢健康肥胖（MHO）。微小RNA（miRNA）可能与MHO有关。这项横断面研究探讨了肥胖情况下循环miRNA与代谢综合征（MetS）主要成分之间的联系。我们还比较了MHO与代谢不健康肥胖（MUO）中的氧化应激生物标志物。我们分析了20名中年肥胖个体的3536种血清miRNA：10名MHO个体和10名MUO个体。共有159种miRNA存在差异表达，其中，MUO组中有72种miRNA（45.2%）表达上调，87种miRNA（54.7%）表达下调。此外，MUO组中与胰岛素信号传导和脂质代谢途径相关的miRNA上调。在这些miRNA中，调节氧化应激的hsa-miR-6796-5p和hsa-miR-4697-3p与血糖、甘油三酯、糖化血红蛋白和高密度脂蛋白胆固醇显著相关。我们的结果提供了根据MetS在肥胖中miRNA差异表达模式的证据，并鉴定了与胰岛素抵抗和脂质代谢途径相关的miRNA。
