Ericson Marna E, Breitschwerdt Edward B, Reicherter Paul, Maxwell Cole, Maggi Ricardo G, Melvin Richard G, Maluki Azar H, Bradley Julie M, Miller Jennifer C, Simmons Glenn E, Dencklau Jamie, Joppru Keaton, Peterson Jack, Bae Will, Scanlon Janet, Bemis Lynne T
T Lab Inc., 910 Clopper Road, Suite 220S, Gaithersburg, MD 20878, USA.
Intracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
Pathogens. 2021 Mar 10;10(3):326. doi: 10.3390/pathogens10030326.
, , and are bacteria known to cause verruga peruana or bacillary angiomatosis, vascular endothelial growth factor (VEGF)-dependent cutaneous lesions in humans. Given the bacteria's association with the dermal niche and clinical suspicion of occult infection by a dermatologist, we determined if patients with melanoma had evidence of spp. infection. Within a one-month period, eight patients previously diagnosed with melanoma volunteered to be tested for evidence of spp. exposure/infection. Subsequently, confocal immunohistochemistry and PCR for spp. were used to study melanoma tissues from two patients. Blood from seven of the eight patients was either seroreactive, PCR positive, or positive by both modalities for spp. exposure. Subsequently, organisms that co-localized with VEGFC immunoreactivity were visualized using multi-immunostaining confocal microscopy of thick skin sections from two patients. Using a co-culture model, was observed to enter melanoma cell cytoplasm and resulted in increased vascular endothelial growth factor C (VEGFC) and interleukin 8 (IL-8) production. Findings from this small number of patients support the need for future investigations to determine the extent to which spp. are a component of the melanoma pathobiome.
巴尔通体、五日热巴通体和汉赛巴尔通体是已知可引起秘鲁疣或杆菌性血管瘤病的细菌，这是人类中依赖血管内皮生长因子（VEGF）的皮肤病变。鉴于这些细菌与皮肤微环境相关，且皮肤科医生临床怀疑存在隐匿感染，我们确定黑色素瘤患者是否有巴尔通体属感染的证据。在一个月的时间内，8名先前被诊断为黑色素瘤的患者自愿接受检测，以寻找巴尔通体属暴露/感染的证据。随后，对两名患者的黑色素瘤组织进行了巴尔通体属的共聚焦免疫组织化学和PCR检测。8名患者中有7名患者的血液对巴尔通体属暴露呈血清反应阳性、PCR阳性或两种检测方式均呈阳性。随后，使用多免疫染色共聚焦显微镜对两名患者的厚皮肤切片进行观察，发现与VEGFC免疫反应共定位的巴尔通体生物。使用共培养模型，观察到巴尔通体进入黑色素瘤细胞胞质，并导致血管内皮生长因子C（VEGFC）和白细胞介素8（IL-8）产生增加。这少数患者的研究结果支持未来需要进行调查，以确定巴尔通体属在多大程度上是黑色素瘤病理生物群落的一个组成部分。
