Felix Stênio Freitas, Rodrigues Alzeir Machado, Rodrigues Ana Livya Moreira, de Freitas José Claudio Carneiro, Alves Daniela Ribeiro, da Silva Alice Araújo, Dos Santos Dayanne Lima, de Oliveira Kethelly Rayne Lima, Montes Renato Almeida, da Silva Marcus Vinicius Ferreira, da Silva Lopes Francisco Flávio, de Morais Selene Maia
Departamento de Ensino, Instituto Federal de Educação, Ciência e Tecnologia do Ceará (IFCE), Campus Iguatu, Rodovia Iguatu/Várzea Alegre, km 05, s/n, Vila Cajazeiras, Iguatu, 63503-790 Ceará, Brazil.
Programa de Pós-Graduação em Biotecnologia, RENORBIO, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, Fortaleza, 60741-000 Ceará, Brazil.
Pharmaceuticals (Basel). 2021 Mar 10;14(3):250. doi: 10.3390/ph14030250.
Insect resistance and environmental pollution are among the drawbacks of continuous use of synthetic insecticides against the vectors of dengue, and . The objective of this study was to analyze the composition of the essential oil of Schauer collected from plants, in three periods of the year, to compare the larvicidal activity and enzymatic inhibition of the dengue vectors. The oilsanalyzed by gas chromatography coupled to mass spectrometry (GC-MS), presented thymol and 1,8-cineole, as the main constituents, in all three periods. This composition was different from that found in previous studies of the species from different places, thus, suggesting a new chemotype of . Larvicidal tests were performed at concentrations of 100, 75, 50, 25, and 12.5 μg.mL and the essential oil from the rainy season showed the best results, with LC of 22.79 μg.mL and 35.36 μg.mL against . and . , respectively; this result was better than other reports. In the rainy period, however, there was a greater variety of components, which led to a better larvicidal effect, possibly due to synergistic action with minor constituents. Total proteins, amylases, and acetylcholinesterase of both species were inhibited by the oils.
持续使用合成杀虫剂对付登革热传播媒介存在诸多弊端，其中包括昆虫抗药性和环境污染。本研究的目的是分析一年中三个时期采集的肖乳香属植物精油的成分，比较其对登革热传播媒介的杀幼虫活性和酶抑制作用。通过气相色谱-质谱联用（GC-MS）分析的精油在所有三个时期均以百里香酚和1,8-桉叶素为主要成分。这种成分与之前对来自不同地方的该物种的研究结果不同，因此表明存在一种新的化学型。在100、75、50、25和12.5μg.mL的浓度下进行了杀幼虫试验，雨季的精油显示出最佳结果，对埃及伊蚊和白纹伊蚊的LC50分别为22.79μg.mL和35.36μg.mL；这一结果优于其他报告。然而，在雨季，成分种类更多，这导致了更好的杀幼虫效果，可能是由于与次要成分的协同作用。两种蚊虫的总蛋白、淀粉酶和乙酰胆碱酯酶均受到精油的抑制。
