富含肌肉的层粘连蛋白相互作用蛋白 (Lamin Interacting Protein, LAP) 结合染色质，是成肌细胞分化所必需的。
Muscle Enriched Lamin Interacting Protein () Binds Chromatin and Is Required for Myoblast Differentiation.
机构信息
Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Molecular Signaling Laboratory, University of Ottawa Heart Institute, Ottawa, ON K1Y 4W7, Canada.
出版信息
Cells. 2021 Mar 10;10(3):615. doi: 10.3390/cells10030615.
Muscle-enriched A-type lamin-interacting protein () is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report 's direct interaction with chromatin, and it may function as a transcriptional co-factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for to associate with genomic regions in close proximity to genes that control tissue-specific differentiation. Gel mobility shift assays confirmed that protein complexes with genomic DNA. Blocking expression in C2C12 myoblasts down-regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that is required for C2C12 myoblast differentiation into myotubes. may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes.
富含肌肉的 A 型层粘连蛋白相互作用蛋白 () 是一种最近发现的羊膜动物基因，编码未知生物学功能的蛋白质。在这里，我们报告了与染色质的直接相互作用，它可能作为转录共因子发挥作用。用微阵列分析进行染色质免疫沉淀表明，倾向于与控制组织特异性分化的基因附近的基因组区域结合。凝胶迁移率变动分析证实了 蛋白与基因组 DNA 的复合物。在 C2C12 成肌细胞中阻断 表达会下调成肌调节因子（MyoD 和 MyoG），随后显著抑制成肌分化和肌管的形成。总的来说，我们的数据表明， 对于 C2C12 成肌细胞分化为肌管是必需的。 可能作为一个独特的羊膜动物的肌发生程序的转录调节剂发挥这种作用。
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