Hefzy Enas Mamdouh, Khalil Mahmoud A F, Amin Amal A Ibrahim, Ashour Hossam M, Abdelaliem Yasser Fathy
Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum 63514, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum 63514, Egypt.
Antibiotics (Basel). 2021 Mar 17;10(3):306. doi: 10.3390/antibiotics10030306.
Probiotics can potentially prevent and treat diseases. We examined the inhibitory activity of bacteriocin-like inhibitory substances (BLISs) from potentially probiotic lactobacilli and streptococci on and non- clinical isolates from women with vulvovaginitis. Using agar well diffusion assays, BLISs inhibited both and non- isolates. The BLIS from isolates had the highest anti- activity (33/45; 73.3%), followed by BLISs from isolates of subsp. (31/45; 68.9%), (30/45; 66.7%) subsp. (30/45; 66.7%), and (30/45; 66.7%). Upon characterization according to the retained activity under variable physical and chemical conditions, the BLISs showed stability against heat, pH, and surfactants, but were protease-sensitive, which suggests a proteinaceous nature of the active substances. Using crystal violet assays, the BLISs reduced the biofilm biomass significantly as compared to a control group that lacked BLISs. In vivo testing of the antagonistic activity was performed using the () larvae model. BLISs significantly improved survival in larvae treated with isolates on the first, second, and seventh days, as compared to larvae inoculated with only ( < 0.01). The results show that BLISs can be used as biotherapeutic agents in vulvovaginal candidiasis.
益生菌具有预防和治疗疾病的潜在能力。我们检测了潜在益生菌乳酸杆菌和链球菌产生的类细菌素抑制物质（BLISs）对来自阴道炎女性的临床和非临床分离株的抑制活性。使用琼脂孔扩散法，BLISs对临床和非临床分离株均有抑制作用。来自[具体菌种1]分离株的BLIS具有最高的抗[目标菌]活性（33/45；73.3%），其次是来自[具体菌种2]亚种[具体菌株2]的分离株（31/45；68.9%）、[具体菌种3]（30/45；66.7%）、[具体菌种4]亚种[具体菌株4]（30/45；66.7%）以及[具体菌种5]（30/45；66.7%）。根据在可变物理和化学条件下保留的活性进行表征时，BLISs对热、pH和表面活性剂表现出稳定性，但对蛋白酶敏感，这表明活性物质具有蛋白质性质。使用结晶紫测定法，与缺乏BLISs的对照组相比，BLISs显著降低了[目标菌]生物膜生物量。使用[具体虫种]（[虫种拉丁名]）幼虫模型进行了拮抗活性的体内测试。与仅接种[目标菌]的幼虫相比，BLISs在第一天、第二天和第七天显著提高了用[目标菌]分离株处理的[具体虫种]幼虫的存活率（P<0.01）。结果表明，BLISs可作为外阴阴道念珠菌病的生物治疗剂。
