基于多糖的复合粒子对固定化姜黄素的稳定性和生物活性的保护作用。

Polysaccharides-Based Complex Particles' Protective Role on the Stability and Bioactivity of Immobilized Curcumin.

机构信息

Department of Pharmaceutical Technology, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 16 University Street, 700115 Iaşi, Romania.

Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Protection of the Environment, Gheorghe Asachi Technical University, 73 Prof. Dr. Docent Dimitrie Mangeron Street, 700050 Iasi, Romania.

出版信息

Int J Mol Sci. 2021 Mar 17;22(6):3075. doi: 10.3390/ijms22063075.

The curcumin degradation represents a significant limitation for its applications. The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors. Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light. The degradation half-life (t) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9. For curcumin extracted from ComPs, t was constant (between 10 and 13 h) and depended on the sample's composition. The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin. Antioxidant activity expressed by IC (µmoles/mL) for FC exposed to UVA decreased by 29%, but curcumin within ComPs was not affected by the UVA. The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree. ComPs have a protective role for the immobilized curcumin.

姜黄素的降解是其应用的一个重大限制。研究了不同多糖基的复合粒子（ComPs）中游离姜黄素（FC）和固定化姜黄素的稳定性，研究了几种因素的作用。紫外可见（UV-VIS）和傅里叶变换红外（FTIR）光谱证明了 FC 的光降解及其作为金属螯合剂的作用：暴露于自然光 28 天后，82%的 FC 和 ComPs 中 26%至 39.79%的姜黄素降解。当 pH 值增加时，FC 的降解半衰期（t）从 pH = 3 时的 6.8 小时减少到 pH = 9 时的 2.1 小时。从 ComPs 中提取的姜黄素的 t 是恒定的（在 10 到 13 小时之间），并且取决于样品的组成。暴露于 λ = 365nm（UVA）的紫外线下的 FC 的总酚（TPC）和总类黄酮（TFC）含量分别增加了 16%和 13%，而固定化姜黄素则没有观察到明显的变化。FC 暴露于 UVA 时，IC（µmoles/mL）表示的抗氧化活性降低了 29%，但 ComPs 中的姜黄素不受 UVA 的影响。ComPs 表面对牛血清白蛋白（BSA）的吸附效率取决于 pH 值和交联度。ComPs 对固定化姜黄素具有保护作用。