Castel M, Morris J F
Department of Zoology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel.
Neuroscience. 1988 Mar;24(3):937-66. doi: 10.1016/0306-4522(88)90078-4.
The oxytocinergic and vasopressinergic innervation of the forebrain of normal mice was studied immunocytochemically by use of a set of mouse monoclonal anti-neurophysins applied to serial vibratome sections. The extensive hypothalamic and extra-hypothalamic location of these neuropeptides was revealed, with, or without colchicine pretreatment. Magnocellular perikarya immunoreactive for either oxytocin-neurophysin or vasopressin-neurophysin were concentrated mainly: in the anterior commissural nucleus; in various subdivisions of the paraventricular nucleus; in a profuse array in the periventricular region; in the supraoptic nucleus including its retrochiasmatic division; in various accessory nuclei; and as a number of cells scattered throughout the preoptic and hypothalamic regions. Extensive groups of parvocellular neurons, containing only vasopressin-neurophysin, were located in the suprachiasmatic nucleus including a ventromedial division, in the bed nucleus of the stria terminalis, and in the medial amygdaloid nucleus. Perikarya in the magnocellular nuclei were of generally similar size distribution and there was no evidence that distinct populations of magnocellular and parvicellular neurons, separable on the basis of size, had been labelled within these nuclei. Within the paraventricular nucleus, however, neurons in the posterior part were smaller than those located more anteriorly, and the cells containing oxytocin-neurophysin were slightly smaller than those containing vasopressin-neurophysin. Within the generally similar size distribution, magnocellular neurons of the anterior commissural nucleus were the largest. During processing, shrinkage of the tissue and immunolabeled cells had occurred. The immunocytochemical procedure delineated neuronal processes, in particular dendrites, very effectively. The dendrites were shown to project for far greater distances than is generally recognized, some were of a characteristic corkscrew-like morphology, and most were oriented in a well-defined pattern. Many dendrites of paraventricular neurons passed medially than caudally towards and then along the third ventricle. Most dendrites of supraoptic neurons, in particular those containing vasopressin-neurophysin, had an extensive anteroposterior course beneath the pia of the base of the brain. The axons containing oxytocin- and vasopressin-neurophysin were shown to take rather different paths from the paraventricular nucleus towards the median eminence.(ABSTRACT TRUNCATED AT 400 WORDS)
利用一组小鼠单克隆抗神经垂体素抗体，对系列振动切片进行免疫细胞化学研究，以观察正常小鼠前脑的催产素能和加压素能神经支配。无论有无秋水仙碱预处理，均揭示了这些神经肽在下丘脑和下丘脑外的广泛分布。对催产素 - 神经垂体素或加压素 - 神经垂体素呈免疫反应的大细胞神经元胞体主要集中在：前连合核；室旁核的各个亚区；室周区域大量分布；视上核包括其视交叉后部分；各种附属核；以及散布在视前区和下丘脑区域的一些细胞。仅含加压素 - 神经垂体素的大量小细胞神经元群位于视交叉上核包括腹内侧部分、终纹床核和杏仁内侧核。大细胞核内的胞体大小分布总体相似，没有证据表明在这些核内基于大小可区分的大细胞和小细胞神经元群体被标记。然而，在室旁核内，后部的神经元比前部的小，含催产素 - 神经垂体素的细胞比含加压素 - 神经垂体素的细胞略小。在前连合核大小总体相似的分布中，大细胞神经元最大。在处理过程中，组织和免疫标记细胞发生了收缩。免疫细胞化学方法非常有效地描绘了神经元突起，特别是树突。树突显示出比通常认为的延伸距离远得多，有些具有特征性的螺旋状形态，并且大多数以明确的模式排列。室旁神经元的许多树突向内侧而非尾侧延伸，朝向并沿着第三脑室。视上神经元的大多数树突，特别是那些含加压素 - 神经垂体素的树突，在脑底部软膜下有广泛的前后走行。含催产素和加压素 - 神经垂体素的轴突从室旁核向正中隆起走行的路径相当不同。（摘要截断于400字）
