Jasinski-Bergner Simon, Blümke Juliane, Wickenhauser Claudia, Seliger Barbara
Institute for Medical Immunology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Magdeburger Straße 2, 06108 Halle (Saale), Germany.
Institute for Pathology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany.
Cancers (Basel). 2021 Mar 9;13(5):1167. doi: 10.3390/cancers13051167.
The two RNA modifications 2'-O-methylation and pseudouridylation occur on several RNA species including ribosomal RNAs leading to an increased translation as well as cell proliferation associated with distinct functions. Using malignant melanoma (MM) as a model system the proteins mediating these RNA modifications were for the first time analyzed by different bioinformatics tools and public available databases regarding their expression and histological localization. Next to this, the impact of these RNA-modifying factors on prognostic relevant processes and marker genes of malignant melanoma was investigated and correlated to immune surveillance and evasion strategies. The RNA modifying factors exerted statistically significant positive correlations to the expression of genes involved in cell proliferation and were statistically significant negative correlated to the expression of human leukocyte antigen class I genes as well as of components of the antigen processing machinery in malignant melanoma. Upregulation of the RNA modifying proteins was of prognostic relevance in this tumor disease with a negative impact on the overall survival of melanoma patients. Furthermore, the expression of known oncogenic miRs, which are induced in malignant melanoma, directly correlated to the expression of factors involved in these two RNA modifications.
两种RNA修饰，即2'-O-甲基化和假尿苷化，发生在包括核糖体RNA在内的多种RNA分子上，导致翻译增加以及与不同功能相关的细胞增殖。以恶性黑色素瘤（MM）为模型系统，首次通过不同的生物信息学工具和公共可用数据库分析了介导这些RNA修饰的蛋白质的表达和组织学定位。除此之外，还研究了这些RNA修饰因子对恶性黑色素瘤预后相关过程和标志物基因的影响，并将其与免疫监视和逃避策略相关联。RNA修饰因子与参与细胞增殖的基因表达呈统计学显著正相关，与恶性黑色素瘤中人类白细胞抗原I类基因以及抗原加工机制成分的表达呈统计学显著负相关。RNA修饰蛋白的上调在这种肿瘤疾病中具有预后相关性，对黑色素瘤患者的总生存期有负面影响。此外，在恶性黑色素瘤中诱导的已知致癌miR的表达与参与这两种RNA修饰的因子的表达直接相关。
