Gómez-Lama Cabanás Carmen, Fernández-González Antonio J, Cardoni Martina, Valverde-Corredor Antonio, López-Cepero Javier, Fernández-López Manuel, Mercado-Blanco Jesús
Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas (CSIC), Campus 'Alameda del Obispo' s/n, Avd. Menéndez Pidal s/n, 14004 Córdoba, Spain.
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Calle Profesor Albareda, 18008 Granada, Spain.
J Fungi (Basel). 2021 Mar 9;7(3):194. doi: 10.3390/jof7030194.
This study aimed to disentangle the structure, composition, and co-occurrence relationships of the banana (cv. Dwarf Cavendish) root endophytome comparing two phenological plant stages: mother plants and suckers. Moreover, a collection of culturable root endophytes (>1000) was also generated from Canary Islands. In vitro antagonism assays against f.sp. () races STR4 and TR4 enabled the identification and characterization of potential biocontrol agents (BCA). Eventually, three of them were selected and evaluated against Fusarium wilt of banana (FWB) together with the well-known BCA PICF7 under controlled conditions. Culturable and non-culturable (high-throughput sequencing) approaches provided concordant information and showed low microbial diversity within the banana root endosphere. appeared as the dominant genus and seemed to play an important role in the banana root endophytic microbiome according to co-occurrence networks. Fungal communities were dominated by the genera and . Overall, significant differences were found between mother plants and suckers, suggesting that the phenological stage determines the recruitment and organization of the endophytic microbiome. While selected native banana endophytes showed clear antagonism against strains, their biocontrol performance against FWB did not improve the outcome observed for a non-indigenous reference BCA (strain PICF7).
本研究旨在剖析香蕉（品种：矮卡文迪什）根内生菌群在母株和吸芽这两个物候期的结构、组成及共生关系。此外，还从加那利群岛分离出了一批可培养的根内生菌（超过1000株）。针对尖孢镰刀菌古巴专化型（Fusarium oxysporum f.sp. cubense）小种STR4和TR4的体外拮抗试验，实现了对潜在生物防治剂（BCA）的鉴定和特性分析。最终，挑选出其中3种与著名的生物防治剂PICF7一起在可控条件下针对香蕉枯萎病（FWB）进行评估。可培养和不可培养（高通量测序）方法提供了一致的信息，并显示香蕉根内圈的微生物多样性较低。根据共生网络，镰刀菌属（Fusarium）似乎是优势属，且在香蕉根内生微生物组中似乎发挥着重要作用。真菌群落以炭疽菌属（Colletotrichum）和盘长孢状刺盘孢菌（C. gloeosporioides）为主。总体而言，母株和吸芽之间存在显著差异，表明物候期决定了内生微生物组的招募和组织。虽然所选的本地香蕉内生菌对尖孢镰刀菌菌株表现出明显的拮抗作用，但其对香蕉枯萎病的生物防治效果并未优于非本土参考生物防治剂（菌株PICF7）。
