Doolabh Kareshma, Naidoo Yougasphree, Dewir Yaser Hassan, Al-Suhaibani Nasser
School of Life Sciences, University of KwaZulu-Natal, Westville, Private Bag X54001, Durban 4000, South Africa.
Plant Production Department, PO Box 2460, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2021 Mar 9;10(3):512. doi: 10.3390/plants10030512.
L. is used as a traditional medicine in treating numerous ailments and diseases such as infertility in women, conjunctivitis, gonorrhea, and jaundice. This study used light and electron microscopy coupled with histochemistry to investigate the micromorphology, ultrastructure and histochemical properties of leaves and stems. Stereo and scanning electron microscopy revealed dense non-glandular trichomes on the leaves and stems and trichome density was greater in emergent leaves than in the young and mature. Three morphologically different non-glandular trichomes were observed including simple multicellular, simple bicellular and simple multicellular hooked. The simple bicellular trichomes were less common than the multicellular and hooked. Transmission electron micrographs showed mitochondria, vesicles and vacuoles in the trichome. The leaf section contained chloroplasts with plastoglobuli and starch grains. Histochemical analysis revealed various pharmacologically important compounds such as phenols, alkaloids, proteins and polysaccharides. The micromorphological and ultrastructural investigations suggest that L. is an economically important medicinal plant due to bioactive compounds present in the leaves and stems.
L. 作为一种传统药物用于治疗多种疾病，如女性不孕症、结膜炎、淋病和黄疸。本研究采用光学显微镜和电子显微镜结合组织化学方法，研究叶片和茎的微观形态、超微结构及组织化学特性。体视显微镜和扫描电子显微镜显示，叶片和茎上有密集的非腺毛，新出叶片上的毛密度大于幼叶和成熟叶。观察到三种形态不同的非腺毛，包括简单多细胞型、简单双细胞型和简单多细胞钩状型。简单双细胞型毛比多细胞型和钩状型少见。透射电子显微镜照片显示毛中有线粒体、囊泡和液泡。叶片切片含有具质体小球和淀粉粒的叶绿体。组织化学分析揭示了多种具有重要药理作用的化合物，如酚类、生物碱、蛋白质和多糖。微观形态和超微结构研究表明，由于叶片和茎中存在生物活性化合物，L. 是一种具有重要经济价值的药用植物。
