Jegal Jonghwan, Chung Ki Wung, Chung Hae Young, Jeong Eun Ju, Yang Min Hye
College of Pharmacy, Pusan National University.
Department of Agronomy & Medicinal Plant Resources, College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology.
Biol Pharm Bull. 2017;40(9):1381-1388. doi: 10.1248/bpb.b17-00122.
In European folk medicine, the fruits of Juniperus communis are used in the treatment of skin-related disorders such as skin infection, itching, and psoriasis. Previously, we reported that the EtOAc fraction of J. communis (EAJC) contained tyrosinase inhibition properties in vitro non-cellular experiment. The aim of this study was to evaluate anti-melanogenic effect of standardized EAJC on a hyperpigmentation animal model. Therapeutic effects of EAJC toward skin hyperpigmentation were confirmed by both in vivo experiment and in vitro cell-based assay. Skin depigmenting effect was detected by topical treatment of EAJC for 11 d to HRM-2 melanin-possessing hairless mice. Histologic findings including significantly decreased melanin depositions could be observed in dorsal skin samples of EAJC-treated group. In addition, the EAJC (50 µg/mL) attenuated melanin production through down-regulation of tyrosinase activity and protein expression in B16 murine melanoma cells. According to the phytochemical analysis, EAJC was found to contain hypolaetin-7-O-β-D-xylopyranoside and isoscutellarein-7-O-β-D-xylopyranoside as main components. Hypolaetin-7-O-β-D-xylopyranoside was responsible for the skin-lightening effect of EAJC by reducing the number of melanocytes in dorsal skins of HRM-2 mice. The present study provided direct experimental evidence for skin-lightening effect of EAJC in UV-irradiated hairless mouse model. Therapeutic attempts with the J. communis might be useful in the management of skin pigmentation-related diseases.
在欧洲民间医学中，杜松子被用于治疗与皮肤相关的疾病，如皮肤感染、瘙痒和牛皮癣。此前，我们报道了杜松子的乙酸乙酯提取物（EAJC）在体外非细胞实验中具有酪氨酸酶抑制特性。本研究的目的是评估标准化EAJC对色素沉着过度动物模型的抗黑素生成作用。通过体内实验和体外细胞实验证实了EAJC对皮肤色素沉着过度的治疗效果。通过对HRM-2黑素生成无毛小鼠局部涂抹EAJC 11天来检测皮肤美白效果。在EAJC处理组的背部皮肤样本中可观察到包括黑色素沉积显著减少在内的组织学结果。此外，EAJC（50µg/mL）通过下调B16小鼠黑色素瘤细胞中酪氨酸酶的活性和蛋白表达来减弱黑色素生成。根据植物化学分析，发现EAJC含有以hypolaetin-7-O-β-D-吡喃木糖苷和异黄芩素-7-O-β-D-吡喃木糖苷为主要成分。Hypolaetin-7-O-β-D-吡喃木糖苷通过减少HRM-2小鼠背部皮肤中黑素细胞的数量来发挥EAJC的美白作用。本研究为EAJC在紫外线照射的无毛小鼠模型中的美白效果提供了直接的实验证据。使用杜松子的治疗尝试可能对皮肤色素沉着相关疾病的治疗有用。
