Vater Claudia, Apanovic Alexandra, Riethmüller Christoph, Litschauer Brigitte, Wolzt Michael, Valenta Claudia, Klang Victoria
Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, University of Vienna, 1090 Vienna, Austria.
Research Platform "Characterisation of Drug Delivery Systems on Skin and Investigation of Involved Mechanisms", University of Vienna, 1090 Vienna, Austria.
Pharmaceutics. 2021 Mar 24;13(4):436. doi: 10.3390/pharmaceutics13040436.
(1) Background: The aim of the study was to evaluate the effect of pure lecithins in comparison to a conventional surfactant on skin in vivo. (2) Methods: Physiological skin parameters were evaluated at the beginning and the end of the study (day 1 and day 4) ( = 8, healthy forearm skin) with an Aquaflux, skin-pH-Meter, Corneometer and an Epsilon sensor. Confocal Raman spectroscopy was employed to monitor natural moisturizing factor, urea and water content of the participants' skin. Tape strips of treated skin sites were taken and the collected corneocytes were subjected to atomic force microscopy. Circular nano objects were counted, and dermal texture indices were determined. (3) Results: Transepidermal water loss was increased, and skin hydration was decreased after treatment with SDS and LPC80. Natural moisturizing factor and urea concentrations within the outermost 10 µm of the stratum corneum were lower than after treatment with S75 or water. Dermal texture indices of skin treated with SDS were higher than skin treated with water (control). (4) Conclusions: Results suggest very good (S75) or good (LPC80) skin-tolerability of lecithin-based surfactants in comparison to SDS and encourage further investigation.
(1) 背景：本研究旨在评估纯卵磷脂与传统表面活性剂相比对体内皮肤的影响。(2) 方法：在研究开始和结束时（第1天和第4天）（n = 8，健康的前臂皮肤），使用皮肤水分流失仪、皮肤pH计、角质层水合度测定仪和ε传感器评估生理皮肤参数。采用共聚焦拉曼光谱法监测参与者皮肤的天然保湿因子、尿素和水分含量。取处理过的皮肤部位的胶带条，对收集的角质形成细胞进行原子力显微镜检查。计数圆形纳米物体，并确定皮肤纹理指数。(3) 结果：用十二烷基硫酸钠（SDS）和1,2-二油酰基磷脂酰胆碱（LPC80）处理后，经表皮水分流失增加，皮肤水合作用降低。角质层最外层10 µm内的天然保湿因子和尿素浓度低于用S75或水处理后的浓度。用SDS处理的皮肤的皮肤纹理指数高于用水处理的皮肤（对照）。(4) 结论：结果表明，与SDS相比，基于卵磷脂的表面活性剂具有非常好的（S75）或良好的（LPC80）皮肤耐受性，并鼓励进一步研究。
