State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling 712100, China.
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China.
Int J Mol Sci. 2021 Mar 24;22(7):3294. doi: 10.3390/ijms22073294.
Jerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions.
Ultra-performance liquid chromatography and tandem mass spectrometry were used to identify differential metabolites in Jerusalem artichoke seedling leaves under polyethylene glycol (PEG) 6000-simulated drought stress at 0, 18, 24, and 36 h.
A total of 661 metabolites and 236 differential metabolites were identified at 0 vs. 18, 18 vs. 24, and 24 vs. 36 h. 146 differential metabolites and 56 common were identified and at 0 vs. 18, 24, and 36 h. Kyoto Encyclopedia of Genes and Genomes enrichment identified 236 differential metabolites involved in the biosynthesis of secondary metabolites and amino acids. Metabolites involved in glycolysis, phenolic metabolism, tricarboxylic cycle, glutamate-mediated proline biosynthesis, urea cycle, amino acid metabolism, unsaturated fatty acid biosynthesis, and the met salvage pathway responded to drought stress.
A metabolic network in the leaves of Jerusalem artichokes under drought stress is proposed. These results will improve understanding of the metabolite response to drought stress in Jerusalem artichokes and develop a foundation for breeding drought-resistant varieties.
洋姜是一种具有高耐旱性和高作为生产生物燃料、功能性饲料和食品原料价值的多年生作物。然而，关于洋姜在干旱条件下的综合代谢组学研究很少。
使用超高效液相色谱和串联质谱法在聚乙二醇（PEG）6000 模拟干旱胁迫下，0、18、24 和 36 h 时鉴定洋姜幼苗叶片中的差异代谢物。
在 0 与 18、18 与 24、24 与 36 h 时，共鉴定出 661 种代谢物和 236 种差异代谢物。在 0 与 18、24 和 36 h 时，鉴定出 146 种差异代谢物和 56 种共同差异代谢物。京都基因与基因组百科全书富集分析鉴定出 236 种参与次生代谢物和氨基酸生物合成的差异代谢物。参与糖酵解、酚类代谢、三羧酸循环、谷氨酸介导的脯氨酸生物合成、尿素循环、氨基酸代谢、不饱和脂肪酸生物合成和甲硫氨酸补救途径的代谢物对干旱胁迫有反应。
提出了干旱胁迫下洋姜叶片中的代谢网络。这些结果将提高对洋姜对干旱胁迫的代谢物响应的理解，并为培育抗旱品种奠定基础。
