Michalik Michał, Kosecka-Strojek Maja, Wolska Mariola, Samet Alfred, Podbielska-Kubera Adrianna, Międzobrodzki Jacek
MML Medical Centre, Bagno 2, 00-112 Warsaw, Poland.
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
Pathogens. 2021 Mar 13;10(3):335. doi: 10.3390/pathogens10030335.
Linezolid is currently used to treat infections caused by multidrug-resistant Gram-positive cocci. Both linezolid-resistant (LRSA) and coagulase-negative staphylococci (CoNS) strains have been collected worldwide. Two isolates carrying linezolid resistance genes were recovered from laryngological patients and characterized by determining their antimicrobial resistance patterns and using molecular methods such as typing, MLST, SCC typing, detection of virulence genes and operon expression, and analysis of antimicrobial resistance determinants. Both isolates were multidrug resistant, including resistance to methicillin. The strain was identified as ST-398/t4474/SCC IVe, harboring adhesin, hemolysin genes, and the operon. The strain was identified as ST-42/-positive and harbored hemolysin genes. Linezolid resistance in strain was associated with the mutations in the ribosomal proteins L3 and L4, and in , resistance was associated with the presence of gene. Moreover, strain harbored and genes. We identified the first case of staphylococci carrying linezolid resistance genes from patients with chronic sinusitis in Poland. Since both and CoNS are the most common etiological factors in laryngological infections, monitoring of such infections combined with surveillance and infection prevention programs is important to decrease the number of linezolid-resistant staphylococcal strains.
利奈唑胺目前用于治疗由多重耐药革兰氏阳性球菌引起的感染。耐利奈唑胺（LRSA）菌株和凝固酶阴性葡萄球菌（CoNS）菌株已在全球范围内被收集。从耳鼻喉科患者中分离出两株携带利奈唑胺耐药基因的菌株，并通过确定其抗菌药物耐药模式以及使用分子方法（如分型、多位点序列分型、葡萄球菌染色体盒式（SCC）分型、毒力基因检测和操纵子表达以及抗菌药物耐药决定因素分析）对其进行了鉴定。两株分离株均对多种药物耐药，包括对甲氧西林耐药。其中一株菌株被鉴定为ST-398/t4474/SCC IVe，携带黏附素、溶血素基因以及该操纵子。另一株菌株被鉴定为ST-42/阳性，携带溶血素基因。第一株菌株中的利奈唑胺耐药与核糖体蛋白L3和L4的突变有关，而在第二株中，耐药与基因的存在有关。此外，第二株菌株还携带了和基因。我们在波兰首次从慢性鼻窦炎患者中鉴定出携带利奈唑胺耐药基因的葡萄球菌病例。由于LRSA和CoNS都是耳鼻喉科感染中最常见的病因，结合监测以及感染预防计划对这类感染进行监测对于减少耐利奈唑胺葡萄球菌菌株的数量很重要。
