Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via D. Montesano 31, I-80131 Naples, Italy.
INDENA SPA, Via Don Minzoni 6, 20090 Settala, Italy.
Biomolecules. 2021 Mar 25;11(4):494. doi: 10.3390/biom11040494.
A madecassoside-rich fraction obtained from the industrial purification of leaves afforded a new triterpene glycoside, named isomadecassoside (), characterized by an ursane-type skeleton and migration of the double bond at Δ in ring E. The structure of isomadecassoside was established by means of HR-ESIMS and detailed analysis of 1D and 2D NMR spectra, which allowed a complete NMR assignment. Studies on isolated J774A.1 macrophages stimulated by LPS revealed that isomadecassoside () inhibited nitrite production at non-cytotoxic concentrations, thus indicating an anti-inflammatory effect similar to that of madecassoside.
从工业纯化的叶子中获得的富含积雪草酸的部分提供了一种新的三萜糖苷，命名为异积雪草酸（），其特征在于五环 E 中双键的Δ迁移和 Ursane 骨架。通过高分辨电喷雾电离质谱（HR-ESIMS）和 1D 和 2D NMR 光谱的详细分析确定了异积雪草酸的结构，从而实现了完整的 NMR 归属。对 LPS 刺激的分离的 J774A.1 巨噬细胞的研究表明，异积雪草酸（）在非细胞毒性浓度下抑制亚硝酸盐的产生，从而表明其具有与积雪草酸相似的抗炎作用。
