Abdou Amr M, Hedia Riham H, Omara Shimaa T, Mahmoud Mohamed Abd El-Fatah, Kandil Mai M, Bakry M A
Department of Microbiology and Immunology, National Research Centre 12622 Dokki, Giza, Egypt.
Department of Parasitology and Animal Diseases, Division of Veterinary Research, National Research Centre, 12622 Dokki, Giza, Egypt.
Vet World. 2018 Feb;11(2):227-230. doi: 10.14202/vetworld.2018.227-230. Epub 2018 Feb 21.
The aim of the current study was to isolate and identify naturally occurring probiotic species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in probiotics on the species level.
A total of 44 fecal and milk samples were collected under aseptic conditions from cattle, buffalo, camel, sheep, goats, and fish. The samples were cultured, and the isolated strains were confirmed biochemically and molecularly using 16S rRNA multiplex polymerase chain reaction (PCR) analysis following DNA extraction from the bacterial isolates.
A total of 31 isolates identified as lactobacilli were isolated from cattle milk, goat feces, sheep feces, fish feces, buffalo milk, camel milk, and goats' milk. species were identified based on the size of the PCR product. The results showed that different species were different in their lactobacilli content. At the same time, there were some differences between individuals of the same species.
The diversity of probiotic strains isolated from different animal species implies different types of benefits to the host. Although it would be both money - and time-consuming research, discovering the benefit of each of these strains may provide very important information for the health of both human and animal. Furthermore, transferring these beneficial effects either to individuals within the same species or between different species would be of great importance.
本研究的目的是分离和鉴定来自不同动物（包括鱼类和农场动物）、具有不同环境背景的天然存在的益生菌物种，以在物种水平上研究益生菌的种间差异。
在无菌条件下，从牛、水牛、骆驼、绵羊、山羊和鱼类中总共采集了44份粪便和乳汁样本。对样本进行培养，从细菌分离物中提取DNA后，使用16S rRNA多重聚合酶链反应（PCR）分析对分离出的菌株进行生化和分子鉴定。
从牛奶、山羊粪便、绵羊粪便、鱼粪便、水牛奶、骆驼奶和羊奶中总共分离出31株被鉴定为乳酸杆菌的菌株。根据PCR产物的大小鉴定物种。结果表明，不同物种的乳酸杆菌含量不同。同时，同一物种的个体之间也存在一些差异。
从不同动物物种分离出的益生菌菌株的多样性意味着对宿主有不同类型的益处。尽管这将是一项既费钱又耗时的研究，但发现这些菌株各自的益处可能会为人类和动物的健康提供非常重要的信息。此外，将这些有益作用转移到同一物种内的个体或不同物种之间将非常重要。
