Seguro Social de Salud del Perú (EsSalud) Andahuaylas, Apurímac 03701, Peru.
Translational Oncology Laboratory, Centro Regional de Investigaciones Biomédicas (CRIB), Universidad de Castilla-La Mancha, 02008 Albacete, Spain.
Nutrients. 2021 Mar 23;13(3):1032. doi: 10.3390/nu13031032.
In diabetes mellitus type 2 (DM2), developed obesity is referred to as diabesity. Implementation of a healthy diet, such as the Mediterranean, prevents diabesity. Saffron is frequently used in this diet because of its bioactive components, such as crocetin (CCT), exhibit healthful properties. It is well known that obesity, defined as an excessive accumulation of fat, leads to cardiometabolic pathology through adiposopathy or hypertrophic growth of adipose tissue (AT).This is related to an impaired adipogenic process or death of adipocytes by obesogenic signals. We aimed to evaluate the effect of the pathogenic microenvironment and CCT, activating differentiation of healthy preadipocytes (PA). For this, we used human cryopreserved PA from visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) depots obtained from healthy and obese-DM2 donors. We studied the effect of a metabolically detrimental (diabesogenic) environment, generated by obese-DM2 adipocytes from VAT (VdDM) or SAT (SdDM), on the viability and accumulation of intracellular fat of adipocytes differentiated from healthy PA, in the presence or absence of CCT (1 or 10 μM). Intracellular fat was quantified by Oil Red O staining. Cytotoxicity was measured using the MTT assay. Our results showed that diabesogenic conditions induce cytotoxicity and provide a proadipogenic environment only for visceral PA. CCT at 10 μM acted as an antiadipogenic and cytoprotective compound.
在 2 型糖尿病（DM2）中，发展肥胖被称为“糖胖病”。实施健康饮食，如地中海饮食，可以预防“糖胖病”。藏红花由于其生物活性成分，如西红花酸（CCT），经常用于这种饮食，因为它具有健康益处。众所周知，肥胖是指脂肪过度积累，通过脂肪病或脂肪组织（AT）的肥大生长导致心脏代谢病理学。这与肥胖信号导致脂肪生成过程受损或脂肪细胞死亡有关。我们旨在评估致病微环境和 CCT 对健康前体脂肪细胞（PA）分化的影响。为此，我们使用了来自健康和肥胖 2 型糖尿病（DM2）供体的内脏脂肪组织（VAT）和皮下脂肪组织（SAT）储存的人类冷冻保存的 PA。我们研究了肥胖 2 型糖尿病（DM2）脂肪细胞（VdDM 或 SdDM）产生的代谢有害（致糖尿病）环境对健康 PA 分化的脂肪细胞的活力和细胞内脂肪积累的影响，同时存在或不存在 CCT（1 或 10 μM）。通过油红 O 染色定量细胞内脂肪。使用 MTT 测定法测量细胞毒性。我们的结果表明，致糖尿病条件诱导细胞毒性并仅为内脏 PA 提供促脂肪生成环境。10 μM 的 CCT 作为一种抗脂肪生成和细胞保护化合物起作用。
