de Block Tessa, Laumen Jolein Gyonne Elise, Van Dijck Christophe, Abdellati Said, De Baetselier Irith, Manoharan-Basil Sheeba Santhini, Van den Bossche Dorien, Kenyon Chris
Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium.
Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, 2000 Antwerp, Belgium.
Pathogens. 2021 Mar 23;10(3):384. doi: 10.3390/pathogens10030384.
In this study, we characterized all oropharyngeal and anorectal isolates of spp. in a cohort of men who have sex with men. This resulted in a panel of pathogenic ( [n = 5] and [n = 5]) and nonpathogenic ( [n = 11], [n = 3] and [n = 2]). A high proportion of strains in this panel were resistant to azithromycin (18/26) and ceftriaxone (3/26). Whole genome sequencing (WGS) of these strains identified numerous mutations that are known to confer reduced susceptibility to azithromycin and ceftriaxone in . The presence or absence of these known mutations did not explain the high level resistance to azithromycin (>256 mg/L) in the nonpathogenic isolates (8/16). After screening for antimicrobial resistance (AMR) genes, we found a ribosomal protection protein, Msr(D), in these highly azithromycin resistant nonpathogenic strains. The complete integration site originated from and is associated with high level resistance to azithromycin in many other bacterial species. This novel AMR resistance mechanism to azithromycin in nonpathogenic could be a public health concern if it were to be transmitted to pathogenic . This study demonstrates the utility of WGS-based surveillance of nonpathogenic .
在本研究中，我们对一组男男性行为者中所有口咽和肛门直肠分离株进行了特征分析。这产生了一组致病性（[n = 5]和[n = 5]）和非致病性（[n = 11]，[n = 3]和[n = 2]）。该组中很大一部分菌株对阿奇霉素（18/26）和头孢曲松（3/26）耐药。对这些菌株进行全基因组测序（WGS）发现了许多已知会导致对阿奇霉素和头孢曲松敏感性降低的突变。这些已知突变的存在与否并不能解释非致病性分离株（8/16）对阿奇霉素的高水平耐药（>256 mg/L）。在筛选抗菌药物耐药（AMR）基因后，我们在这些对阿奇霉素高度耐药的非致病性菌株中发现了一种核糖体保护蛋白Msr(D)。完整的整合位点源自，并且与许多其他细菌物种对阿奇霉素的高水平耐药有关。如果这种非致病性的对阿奇霉素的新型AMR耐药机制传播给致病性，可能会成为一个公共卫生问题。本研究证明了基于WGS监测非致病性的实用性。
