de Block Tessa, González Natalia, Abdellati Saïd, Laumen Jolein Gyonne Elise, Van Dijck Christophe, De Baetselier Irith, Van den Bossche Dorien, Manoharan-Basil Sheeba S, Kenyon Chris
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Laboratory of Medical Microbiology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
Front Microbiol. 2022 Mar 30;13:855482. doi: 10.3389/fmicb.2022.855482. eCollection 2022.
Resistance acquisition natural transformation is a common process in the genus. Transformation has played an important role in the emergence of resistance to many antimicrobials in and . In a previous study, we found that currently circulating isolates of had acquired an gene that has been found to result in macrolide resistance in other bacteria but never found in species before. To determine if this resistance mechanism is transferable among species, we assessed if we could transform the gene into other commensal and pathogenic under low dose azithromycin pressure. Intraspecies recombination in commensal was confirmed with PCR and resulted in high-level macrolide resistance. Whole-genome sequencing of these transformed strains identified the complete uptake of the integration fragment. Sequence analysis showed that a large fragment of DNA (5 and 12 kb) was transferred through a single horizontal gene transfer event. Furthermore, uptake of the gene had no apparent fitness cost. Interspecies transformation of from to was, however, not successful.
耐药性获得 自然转化是该属中的常见过程。转化在[具体物种1]和[具体物种2]对多种抗菌药物耐药性的出现中发挥了重要作用。在先前的一项研究中，我们发现目前流行的[具体物种1]分离株获得了一个[基因名称]基因，该基因已被发现会导致其他细菌产生大环内酯类耐药性，但此前从未在[具体物种1]物种中发现过。为了确定这种耐药机制是否可在[具体物种1]物种间转移，我们评估了在低剂量阿奇霉素压力下，是否能够将[基因名称]基因转化到其他共生和致病性[具体物种1]中。通过PCR证实了共生[具体物种1]中的种内重组，并导致了高水平的大环内酯类耐药性。对这些转化菌株的全基因组测序确定了[基因名称]整合片段的完整摄取。序列分析表明，一大段DNA（5和12 kb）通过单个水平基因转移事件进行了转移。此外，摄取[基因名称]基因没有明显的适应性代价。然而，从[具体物种1]到[具体物种2]的种间转化并不成功。
