Dong Huan Vinh, Adamson Paul, Pham Dieu Hoa, Pham Quynh Hoa, Le Hai Ha Long, Pham Loc Quang, Bui Hao Thi Minh, Le Giang Minh, Klausner Jeffrey D
Division of Pediatric Infectious Diseases, David Geffen School of Medicine at University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
J Glob Antimicrob Resist. 2025 Jan;40:11-14. doi: 10.1016/j.jgar.2024.11.008. Epub 2024 Nov 28.
Antimicrobial resistance (AMR) in Neisseria gonorrhoeae (N. gonorrhoeae) is an urgent global health concern. Commensal Neisseria species in the oropharynx are an important reservoir of AMR genes that are transferred to N. gonorrhoeae, yet few data about AMR among commensal Neisseria in populations at risk for AMR exist.
From May 2022 to December 2023, men in an HIV pre-exposure prophylaxis program in Hanoi, Vietnam, were recruited for this study. Participants self-collected oral specimens using phosphate buffer solution, for culture on LB agar media containing sucrose, vancomycin, and trimethoprim (LBVT.SNR). Oxidase-positive Gram-negative diplococci were identified using the Remel RapID NH system. Minimum inhibitory concentrations (MICs) to azithromycin, ceftriaxone, cefixime, and doxycycline were determined using Etests.
There were 42 male participants, the median age was 26 years and 29% (n = 12) reported using antibiotics in the past 6 months. In total, 48 Neisseria isolates were recovered; N. sicca/subflava was the most common species (50%; n = 24), followed by N. mucosa (38%; n = 18). For azithromycin, 85% (n = 41) of isolates were resistant with MICs ≥ 1 ug/mL, including 25% (n = 12) with high-level resistance (MICs ≥ 256 ug/mL of which 67% (8/12) were N. mucosa. Among non-gonococcal Neisseria isolates, the prevalence of resistance was 6% (n = 3) for ceftriaxone, 6% (n = 3) for cefixime, and 54% (n = 26) for doxycycline; the most non-susceptible isolates were N. mucosa.
A High frequency of azithromycin resistance, moderate doxycycline resistance, and low cephalosporin resistance was found in oropharyngeal Neisseria isolates from men who have sex with men (MSM) in a pre-exposure prophylaxis (PrEP) program in Hanoi, Vietnam. N. mucosa was over-represented among resistant isolates.
淋病奈瑟菌（N. gonorrhoeae）的抗菌药物耐药性（AMR）是一个紧迫的全球卫生问题。口咽部的共生奈瑟菌属是AMR基因的重要储存库，这些基因会转移到淋病奈瑟菌中，但关于有AMR风险人群中共生奈瑟菌属的AMR数据很少。
2022年5月至2023年12月，越南河内一个HIV暴露前预防项目中的男性被招募参加本研究。参与者使用磷酸盐缓冲溶液自行采集口腔标本，用于在含有蔗糖、万古霉素和甲氧苄啶的LB琼脂培养基（LBVT.SNR）上培养。使用Remel RapID NH系统鉴定氧化酶阳性革兰氏阴性双球菌。使用Etest测定对阿奇霉素、头孢曲松、头孢克肟和多西环素的最低抑菌浓度（MIC）。
有42名男性参与者，中位年龄为26岁，29%（n = 12）报告在过去6个月内使用过抗生素。总共分离出48株奈瑟菌；微黄奈瑟菌/干燥奈瑟菌是最常见的菌种（50%；n = 24），其次是黏膜奈瑟菌（38%；n = 18）。对于阿奇霉素，85%（n = 41）的分离株耐药，MIC≥1μg/mL，并包括25%（n = 12）的高水平耐药（MIC≥256μg/mL，其中67%（8/12）为黏膜奈瑟菌）。在非淋菌性奈瑟菌分离株中，头孢曲松的耐药率为6%（n = 3），头孢克肟为6%（n = 3），多西环素为54%（n = 26）；最不易感的分离株是黏膜奈瑟菌。
在越南河内一个暴露前预防（PrEP）项目中，与男性发生性关系者（MSM）的口咽部奈瑟菌分离株中发现了高频率的阿奇霉素耐药、中度多西环素耐药和低水平头孢菌素耐药。耐药分离株中黏膜奈瑟菌的比例过高。
