Nasirzade Jila, Alccayhuaman Karol Alí Apaza, Kargarpour Zahra, Kuchler Ulrike, Strauss Franz Josef, Panahipour Layla, Kampleitner Carina, Heimel Patrick, Schwarz Frank, Gruber Reinhard
Department of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria.
Department of Oral Surgery, Medical University of Vienna, 1090 Vienna, Austria.
Biology (Basel). 2021 Mar 5;10(3):196. doi: 10.3390/biology10030196.
Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since tooth roots undergo creeping substitution similar to bone grafts, it can be hypothesized that osteoclasts release the growth factors stored in the dentin thereby influencing bone formation. To test this hypothesis, collagen membranes were either soaked in acid dentin lysates (ADL) from extracted porcine teeth or serum-free medium followed by lyophilization. Thereafter, these membranes covered standardized 5-mm-diameter critical-size defects in calvarial bone on rats. After four weeks of healing, micro-computed tomography and histological analyses using undecalcified thin ground sections were performed. Micro-computed tomography of the inner 4.5 mm calvaria defects revealed a median bone defect coverage of 91% (CI: 87-95) in the ADL group and 94% (CI: 65-100) in the control group, without significant differences between the groups (intergroup > 0.05). Furthermore, bone volume (BV) was similar between ADL group (5.7 mm, CI: 3.4-7.1) and control group (5.7 mm, CI: 2.9-9.7). Histomorphometry of the defect area confirmed these findings with bone area values amounting to 2.1 mm (CI: 1.2-2.6) in the ADL group and 2.0 mm (CI: 1.1-3.0) in the control group. Together, these data suggest that acid dentin lysate lyophilized onto collagen membranes failed to modulate the robust bone formation when placed onto calvarial defects.
自体牙根作为一种移植材料越来越多地应用于牙槽骨增量手术中。由于牙根会经历类似于骨移植的爬行替代过程，因此可以推测破骨细胞会释放储存于牙本质中的生长因子，从而影响骨形成。为验证这一假设，将胶原膜浸泡于拔除的猪牙的酸性牙本质裂解液（ADL）或无血清培养基中，随后冻干。之后，将这些膜覆盖于大鼠颅骨上直径为5 mm的标准化临界尺寸骨缺损处。愈合四周后，采用未脱钙薄磨片进行显微计算机断层扫描和组织学分析。对颅骨缺损内部4.5 mm的显微计算机断层扫描显示，ADL组骨缺损的中位覆盖率为91%（CI：87 - 95），对照组为94%（CI：65 - 100），两组间无显著差异（组间P>0.05）。此外，ADL组（5.7 mm，CI：3.4 - 7.1）和对照组（5.7 mm，CI：2.9 - 9.7）的骨体积（BV）相似。缺损区域的组织形态计量学分析证实了这些结果，ADL组的骨面积值为2.1 mm（CI：1.2 - 2.6），对照组为2.0 mm（CI：1.1 - 3.0）。总之，这些数据表明，冻干在胶原膜上的酸性牙本质裂解液置于颅骨缺损处时，未能调节强劲的骨形成。
