Agricultural College, Guangxi University, Nanning 530005, China.
College of Chinese Materia Medica, Guangdong Food and Drug Vocational College, Guangzhou 510520, China.
Molecules. 2018 Aug 13;23(8):2014. doi: 10.3390/molecules23082014.
Simple sequence repeats (SSRs) or microsatellite markers derived from expressed sequence tags (ESTs) are routinely used for molecular assisted-selection breeding, comparative genomic analysis, and genetic diversity studies. In this study, we investigated 54,546 ESTs for the identification and development of SSR markers in (Patchouli). In total, 1219 SSRs were identified from 1144 SSR-containing ESTs. Trinucleotides (80.8%) were the most abundant SSRs, followed by di- (10.8%), mono- (7.1%), and hexa-nucleotides (1.3%). The top six motifs were CCG/CGG (15.3%), AAG/CTT (15.0%), ACC/GGT (13.5%), AGG/CCT (12.4%), ATC/ATG (9.9%), and AG/CT (9.8%). On the basis of these SSR-containing ESTs, a total of 192 primer pairs were randomly designed and used for polymorphism analysis in 38 accessions collected from different geographical regions of Guangdong, China. Of the SSR markers, 45 were polymorphic and had allele variations from two to four. Furthermore, a transferability analysis of these primer pairs revealed a 10⁻40% cross-species transferability in 10 related species. This report is the first comprehensive study on the development and analysis of a large set of SSR markers in . These markers have the potential to be used in quantitative trait loci mapping, genetic diversity studies, and the fingerprinting of cultivars of
简单序列重复（SSR）或微卫星标记物源自表达序列标签（EST），常用于分子辅助选择育种、比较基因组分析和遗传多样性研究。本研究中，我们对 54546 条 EST 进行了调查，以鉴定和开发 （广藿香）中的 SSR 标记物。共从 1144 条含 SSR 的 EST 中鉴定出 1219 个 SSR。三核苷酸（80.8%）是最丰富的 SSR，其次是二核苷酸（10.8%）、单核苷酸（7.1%）和六核苷酸（1.3%）。前六个基序为 CCG/CGG（15.3%）、AAG/CTT（15.0%）、ACC/GGT（13.5%）、AGG/CCT（12.4%）、ATC/ATG（9.9%）和 AG/CT（9.8%）。基于这些含 SSR 的 EST，共设计了 192 对引物，随机用于来自中国广东不同地理区域的 38 个品种的多态性分析。在这些 SSR 标记物中，有 45 个具有多态性，等位基因变异数从两个到四个。此外，这些引物对的可转移性分析显示，在 10 种相关物种中有 10⁻40%的跨物种可转移性。本报告是首次对 （广藿香）中大量 SSR 标记物的开发和分析进行的全面研究。这些标记物有可能用于数量性状基因座作图、遗传多样性研究和品种指纹图谱分析。
