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对巴西苏木和罗望子种子产生的真菌木质纤维素酶的展望:生物反应器规模扩大及甘蔗渣糖化特性

Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba () and Tamarind () Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse.

作者信息

Contato Alex Graça, de Oliveira Tássio Brito, Aranha Guilherme Mauro, de Freitas Emanuelle Neiverth, Vici Ana Claudia, Nogueira Karoline Maria Vieira, de Lucas Rosymar Coutinho, Scarcella Ana Sílvia de Almeida, Buckeridge Marcos Silveira, Silva Roberto Nascimento, Polizeli Maria de Lourdes Teixeira de Moraes

机构信息

Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, Brazil.

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14050-901, Brazil.

出版信息

Microorganisms. 2021 Mar 5;9(3):533. doi: 10.3390/microorganisms9030533.

Abstract

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using and seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: , , sp., two strains of , , sp. and . The best producers concerning enzymatic activity were and . The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.

摘要

木质纤维素生物质由三种主要成分组成

纤维素、半纤维素和木质素。这三种成分的降解和转化对生物技术具有吸引力。本研究旨在通过使用[具体物质1]和[具体物质2]种子作为碳源进行时间分析,来探寻具有潜在工业应用价值的真菌木质纤维素分解酶。测定了α-L-阿拉伯呋喃糖苷酶、乙酰木聚糖酯酶、内切-1,5-α-L-阿拉伯聚糖酶、β-D-半乳糖苷酶、β-D-葡萄糖苷酶、β-葡聚糖酶、β-D-木糖苷酶、纤维二糖水解酶、内切葡聚糖酶、地衣芽孢杆菌酶、甘露聚糖酶、聚半乳糖醛酸酶、内切-1,4-β-木聚糖酶和木葡聚糖酶的活性。针对8种丝状真菌产生了这些酶:[真菌名称1]、[真菌名称2]、[真菌名称3]种、两株[真菌名称4]、[真菌名称5]、[真菌名称6]种和[真菌名称7]。在酶活性方面最佳的产生菌是[真菌名称8]和[真菌名称9]。酶产生的最佳条件是在搅拌下添加罗望子种子的培养基,培养72小时。该分析对于证明培养条件(静态和搅拌)对不同真菌产生的几种酶的产生有强烈影响至关重要。甘蔗的种类、使用的预处理、微生物和碳源被证明是限制糖谱的因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9123/8000596/d78996fc188e/microorganisms-09-00533-g001.jpg

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