Böswald Linda F, Matzek Dana, Kienzle Ellen, Popper Bastian
Department of Animal Nutrition and Dietetics, Ludwig-Maximilians-Universität München, Schönleutnerstr. 8, 85764 Oberschleißheim, Germany.
Biomedical Center, Core Facility Animal Models, Faculty of Medicine, Ludwig-Maximilians-Universität Munich, Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany.
Animals (Basel). 2021 Mar 5;11(3):702. doi: 10.3390/ani11030702.
Acid base homeostasis and urine pH is influenced by the dietary cation anion balance (DCAB) in many species. Here, a negative DCAB acidifies the urine, while higher DCABs alkalize the urine. The dimension of the DCAB effect can be species-specific, because of differences in urine buffer systems. The aim of the present study was to describe the response of laboratory mice to diets with different DCAB. We used 8-week-old wildtype male mice of the C57Bl/6J inbred strain and CD1 outbred stock. Three groups (n = 15 animals/group) were formed and fed standard diet A for adaptation. For the 7-week feeding trial, mice were either kept on diet A (DCAB -7 mmol/kg dry matter (DM) or switched to diet B (246 mmol/kg DM) or C (-257 mmol/kg DM). Urine pH was measured weekly from a pooled sample per cage. There was a significant difference in the basal urine pH on diet A between C57Bl6/J and CD1 mice. The shift in urine pH was also significantly different between the two groups investigated.
在许多物种中，酸碱平衡和尿液pH值受饮食阴阳离子平衡（DCAB）的影响。在此，负DCAB会使尿液酸化，而较高的DCAB会使尿液碱化。由于尿液缓冲系统的差异，DCAB效应的程度可能因物种而异。本研究的目的是描述实验室小鼠对不同DCAB饮食的反应。我们使用了8周龄的C57Bl/6J近交系野生型雄性小鼠和CD1远交系小鼠。将其分成三组（每组n = 15只动物），并喂食标准饮食A以进行适应。在为期7周的喂养试验中，小鼠要么继续食用饮食A（DCAB为-7 mmol/kg干物质（DM）），要么换成饮食B（246 mmol/kg DM）或C（-257 mmol/kg DM）。每周从每个笼子的混合样本中测量尿液pH值。C57Bl6/J小鼠和CD1小鼠在饮食A时的基础尿液pH值存在显著差异。在研究的两组之间，尿液pH值的变化也存在显著差异。
