Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia.
Kotel'nikov Institute of Radio Engineering and Electronics of RAS, Saratov Branch, Saratov 410019, Russia.
Sensors (Basel). 2021 Mar 5;21(5):1822. doi: 10.3390/s21051822.
A method for the rapid detection of coronaviruses is presented on the example of the transmissible gastroenteritis virus (TGEV) directly in aqueous solutions with different conductivity. An acoustic sensor based on a slot wave in an acoustic delay line was used for the research. The addition of anti-TGEV antibodies (Abs) diluted in an aqueous solution led to a change in the depth and frequency of resonant peaks on the frequency dependence of the insertion loss of the sensor. The difference in the output parameters of the sensor before and after the biological interaction of the TGE virus in solutions with the specific antibodies allows drawing a conclusion about the presence/absence of the studied viruses in the analyzed solution. The possibility for virus detection in aqueous solutions with the conductivity of 1.9-900 μs/cm, as well as in the presence of the foreign viral particles, has been demonstrated. The analysis time did not exceed 10 min.
本文提出了一种基于声延迟线中缝隙波的声学传感器，可直接在具有不同电导率的水溶液中快速检测冠状病毒，以传染性胃肠炎病毒（TGEV）为例。研究中使用了一种基于声延迟线中缝隙波的声学传感器。在水溶液中稀释的抗 TGEV 抗体（Abs）的加入导致传感器插入损耗频率依赖关系上的共振峰深度和频率发生变化。在具有特定抗体的溶液中进行 TGE 病毒的生物相互作用前后传感器输出参数的差异，可以得出关于分析溶液中是否存在研究病毒的结论。已经证明了在电导率为 1.9-900 μs/cm 的水溶液中和在存在外源病毒颗粒的情况下检测病毒的可能性。分析时间不超过 10 分钟。
