Council for Agricultural Research and Economics (CREA), Research Centre for Olive, Fruit and Citrus Crops, 95024 Acireale, Italy.
Department of Agricultural, Food and Environment, University of Catania, 95123 Catania, Italy.
Molecules. 2021 Mar 30;26(7):1944. doi: 10.3390/molecules26071944.
Olive mill wastewater (OMW) contains valuable and interesting bioactive compounds, among which is hydroxytyrosol, which is characterized by a remarkable antioxidant activity. Due to the health claims related to olive polyphenols, the aim of this study was to obtain an extract from OMW with an increased level of hydroxytyrosol by means of microbial enzymatic activity. For this purpose, four commercial adsorbent resins were selected and tested. The beta-glucosidase and esterase activity of strains of , and were also investigated and compared to those of a commercial enzyme and an strain. The strain showed the best enzymatic performances. The SP207 resin showed the best efficiency in selective recovery of hydroxytyrosol, tyrosol, oleuropein, and total phenols. The bioconversion test of the OMW extract was assessed by using both culture broths and pellets of the tested strains. The results demonstrated that the pellets of and were the most effective in hydroxytyrosol increasing in phenolic extract. The interesting results suggest the possibility to study new formulations of OMW phenolic extracts with multifunctional microorganisms.
橄榄油厂废水 (OMW) 含有有价值且有趣的生物活性化合物，其中包括羟基酪醇，其具有显著的抗氧化活性。由于与橄榄多酚相关的健康声称，本研究旨在通过微生物酶活性从 OMW 中获得羟基酪醇含量增加的提取物。为此，选择并测试了四种商业吸附树脂。还研究和比较了 、 和 菌株的β-葡萄糖苷酶和酯酶活性与商业酶和一株 菌株的活性。 菌株表现出最佳的酶性能。SP207 树脂在选择性回收羟基酪醇、酪醇、橄榄苦苷和总酚方面表现出最佳效率。使用测试菌株的培养液和菌球评估了 OMW 提取物的生物转化试验。结果表明， 和 菌球在增加酚类提取物中的羟基酪醇方面最为有效。有趣的结果表明，有可能研究具有多功能微生物的 OMW 酚类提取物的新配方。
