Štambuk Petra, Šikuten Iva, Preiner Darko, Nimac Ana, Lazarević Boris, Marković Zvjezdana, Maletić Edi, Kontić Jasminka Karoglan, Tomaz Ivana
Department of Viticulture and Enology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia.
Centre of Excellence for Biodiversity and Molecular Plant Breeding, Svetošimunska cesta 25, 10000 Zagreb, Croatia.
Plants (Basel). 2021 Mar 30;10(4):661. doi: 10.3390/plants10040661.
In the era of sustainable grapevine production, there is a growing demand to define differences between varieties in susceptibility to downy mildew. Croatia, as a country with a long tradition of grapevine cultivation, preserves a large number of native grapevine varieties. A leaf disc bioassay has been conducted on 25 of them to define their response to downy mildew, according to the International Organisation of Vine and Wine (OIV) descriptor 452-1, together with the stress response of the leaf discs using chlorophyll fluorescence and multispectral imaging with 11 parameters included. Time points of measurement were as follows: before treatment (T), one day post-inoculation (dpi) (T), two dpi (T), three dpi (T), four dpi (T), six dpi (T), and eight dpi (T). Visible changes in form of developed () sporulation were evaluated on the seventh day upon inoculation. Results show that methods applied here distinguish varieties of different responses to downy mildew. Based on the results obtained, a phenotyping model in the absence of the pathogen is proposed, which is required to confirm by conducting more extensive research.
在可持续葡萄生产的时代，明确不同葡萄品种对霜霉病易感性的差异的需求日益增长。克罗地亚作为一个有着悠久葡萄种植传统的国家，保留了大量本土葡萄品种。根据国际葡萄与葡萄酒组织（OIV）描述符452-1，对其中25个品种进行了叶盘生物测定，以确定它们对霜霉病的反应，同时利用叶绿素荧光和包含11个参数的多光谱成像来测定叶盘的应激反应。测量时间点如下：处理前（T0）、接种后1天（T1）、2天（T2）、3天（T3）、4天（T4）、6天（T6）和8天（T8）。在接种后第七天评估可见的（霜霉病）孢子形成形式的变化。结果表明，这里应用的方法能够区分对霜霉病有不同反应的品种。基于所获得的结果，提出了一个在无病原体情况下的表型模型，这需要通过开展更广泛的研究来证实。
