Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, D-01307 Dresden, Germany.
Faculty of Sustainability, Institute of Sustainable and Environmental Chemistry, Leuphana University Lueneburg, Universitaetsallee. 1, C13, 21335 Lueneburg, Germany.
Nutrients. 2021 Mar 19;13(3):989. doi: 10.3390/nu13030989.
Lipids are of interest for the targeted modification of oral bioadhesion processes. Therefore, the sustainable effects of linseed oil on the composition and ultrastructure of the in situ pellicle were investigated. Unlike saliva, linseed oil contains linolenic acid (18:3), which served as a marker for lipid accumulation. Individual splints with bovine enamel slabs were worn by five subjects. After 1 min of pellicle formation, rinses were performed with linseed oil for 10 min, and the slabs' oral exposure was continued for up to 2 or 8 h. Gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS) was used to characterize the fatty acid composition of the pellicle samples. Transmission electron microscopy was performed to analyze the ultrastructure. Extensive accumulation of linolenic acid was recorded in the samples of all subjects 2 h after the rinse and considerable amounts persisted after 8 h. The ultrastructure of the 2 h pellicle was less electron-dense and contained lipid vesicles when compared with controls. After 8 h, no apparent ultrastructural effects were visible. Linolenic acid is an excellent marker for the investigation of fatty acid accumulation in the pellicle. New preventive strategies could benefit from the accumulation of lipid components in the pellicle.
脂质是靶向修饰口腔生物黏附过程的研究对象。因此，研究了亚麻籽油对原位黏膜层的组成和超微结构的持续影响。与唾液不同，亚麻籽油中含有亚麻酸（18:3），可作为脂质积累的标志物。五位受试者佩戴带有牛牙釉质片的个体夹板。在形成黏膜层 1 分钟后，用亚麻籽油冲洗 10 分钟，然后继续使牙釉质片在口腔中暴露长达 2 或 8 小时。采用气相色谱-电子轰击电离质谱联用（GC-EI/MS）技术对黏膜层样本的脂肪酸组成进行了分析。采用透射电子显微镜分析了超微结构。冲洗后 2 小时，所有受试者样本中均记录到大量的亚麻酸积累，8 小时后仍有大量积累。与对照组相比，2 小时黏膜层的超微结构电子密度较低，并且含有脂质小泡。8 小时后，未观察到明显的超微结构影响。亚麻酸是研究黏膜层中脂肪酸积累的理想标志物。新的预防策略可能受益于脂质成分在黏膜层中的积累。
